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Catalysts 2017, 7(6), 188; doi:10.3390/catal7060188

Using Laccases in the Nanoflower to Synthesize Viniferin

1
College of Life Science, Jilin Agricultural University, Changchun 130118, China
2
Informatization Center for Education and Management, Jilin Agricultural University, Changchun 130118, China
3
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China
*
Authors to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 30 March 2017 / Revised: 24 May 2017 / Accepted: 1 June 2017 / Published: 12 June 2017
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Abstract

The laccase-incorporated nanoflower was fabricated and characterized by scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). SEM images indicate that the laccase-incorporated nanoflower has a high surface area, which may facilitate the mass transfer of the substrate and the product. FTIR spectrums identify the existence of laccase in the nanoflowers. The novel immobilized laccase was used for the synthesis of viniferin. The reaction conditions had been optimized and the laccase-incorporated nanoflower can show its maximum specific activity (16.3 µmol/g/h) under the optimal reaction conditions. The specific activity of the laccase in the nanoflowers is enhanced about 2.2-fold compared with free laccase in solution without copper (II) ions. Furthermore, the laccase in the nanoflowers shows an increase in specific activity of ~180% compared with free laccase in a solution containing high concentrations (similar to the concentration in the flower) of copper (II) ions. The results also indicate that the laccase in the nanoflowers retain 93.2% of its initial specific activity even after ten continuous batches. View Full-Text
Keywords: laccase; nanoflower; viniferin laccase; nanoflower; viniferin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wu, Z.; Li, H.; Zhu, X.; Li, S.; Wang, Z.; Wang, L.; Li, Z.; Chen, G. Using Laccases in the Nanoflower to Synthesize Viniferin. Catalysts 2017, 7, 188.

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