Stabilization of a Lipolytic Enzyme for Commercial Application
AbstractThermomyces lanouginosa lipase has been used to develop improved methods for carrier-free immobilization, the Cross-Linked Enzyme Aggregates (CLEAs), for its application in detergent products. An activator step has been introduced to the CLEAs preparation process with the addition of Tween 80 as activator molecule, in order to obtain a higher number of the individual lipase molecules in the ”open lid” conformation prior to the cross-linking step. A terminator step has been introduced to quench the cross-linking reaction at an optimal time by treatment with an amine buffer in order to obtain smaller and more homogenous cross-linked particles. This improved immobilization method has been compared to a commercially available enzyme and has been shown to be made up of smaller and more homogenous particles with an average diameter of 1.85 ± 0.28 µm which are 129.7% more active than the free enzyme. The CLEAs produced show improved features for commercial applications such as an improved wash performance comparable with the free enzyme, improved stability to proteolysis and a higher activity after long-term storage. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
De Rose, S.A.; Novak, H.; Dowd, A.; Singh, S.; Lang, D.A.; Littlechild, J. Stabilization of a Lipolytic Enzyme for Commercial Application. Catalysts 2017, 7, 91.
De Rose SA, Novak H, Dowd A, Singh S, Lang DA, Littlechild J. Stabilization of a Lipolytic Enzyme for Commercial Application. Catalysts. 2017; 7(3):91.Chicago/Turabian Style
De Rose, Simone Antonio; Novak, Halina; Dowd, Andrew; Singh, Sukriti; Lang, Dietmar Andreas; Littlechild, Jennifer. 2017. "Stabilization of a Lipolytic Enzyme for Commercial Application." Catalysts 7, no. 3: 91.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.