Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of the Calibration Set and Prediction Set by AAT
2.2. Collection NIRS Data
2.3. Germination Test
2.4. Development and Cross-Validation of the NIRS Calibration Model
2.5. External Validation of the NIRS Calibration Model
3. Results
3.1. Preparation of the Calibration Set and Prediction Set by AAT
3.2. Development and Cross-Validation of the NIRS Calibration Model
3.3. External Validation
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Regressio Method z | GH y | Math Treatment | N x | Mean w | SD v | Calibration u | Cross-Validation t | |||
---|---|---|---|---|---|---|---|---|---|---|
SEC | RSQ | SECV | 1-VR | SD/SECV | ||||||
MPLS | 10 | 1,4,4,1 | 260 | 70.58 | 27.49 | 6.6119 | 0.9421 | 7.8291 | 0.9187 | 3.51 |
3 | 1,4,4,1 | 256 | 70.72 | 27.61 | 6.5012 | 0.9446 | 7.8264 | 0.9194 | 3.53 | |
3 | 2,4,4,1 | 255 | 70.93 | 27.23 | 6.5515 | 0.9421 | 8.2729 | 0.9074 | 3.29 | |
3 | 2,8,6,1 | 256 | 70.60 | 27.45 | 6.6374 | 0.9416 | 8.0404 | 0.9139 | 3.41 | |
PLS | 10 | 1,4,4,1 | 260 | 70.06 | 27.68 | 7.7336 | 0.9220 | 8.5802 | 0.9036 | 3.23 |
3 | 1,4,4,1 | 259 | 70.20 | 27.65 | 7.7901 | 0.9206 | 8.7277 | 0.9001 | 3.17 | |
3 | 2,4,4,1 | 258 | 70.16 | 27.32 | 8.6306 | 0.9002 | 10.175 | 0.8608 | 2.69 | |
3 | 2,8,6,1 | 258 | 70.38 | 27.52 | 7.9458 | 0.9167 | 9.2901 | 0.8857 | 2.96 | |
PCR | 10 | 1,4,4,1 | 262 | 69.87 | 27.71 | 13.0589 | 0.7779 | 13.0699 | 0.7769 | 2.12 |
3 | 1,4,4,1 | 261 | 69.76 | 27.70 | 13.0164 | 0.7792 | 13.0323 | 0.7780 | 2.13 | |
3 | 2,4,4,1 | 260 | 69.92 | 27.59 | 12.8153 | 0.7843 | 13.1216 | 0.7733 | 2.10 | |
3 | 2,8,6,1 | 259 | 69.70 | 27.57 | 14.5249 | 0.7225 | 14.5353 | 0.7212 | 1.90 |
GH z | N | Mean (P) | Mean (M) | SD (P) | SD (M) | Bias | Slope | R2 | SEP | SEP (C) | RPD |
---|---|---|---|---|---|---|---|---|---|---|---|
3 | 100 | 70.89 | 70.26 | 28.12 | 27.46 | 0.626 | 0.992 | 0.939 | 6.93 | 6.57 | 3.96 |
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Lee, H.-S.; Jeon, Y.-A.; Lee, Y.-Y.; Lee, G.-A.; Raveendar, S.; Ma, K.H. Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS). Sustainability 2017, 9, 618. https://doi.org/10.3390/su9040618
Lee H-S, Jeon Y-A, Lee Y-Y, Lee G-A, Raveendar S, Ma KH. Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS). Sustainability. 2017; 9(4):618. https://doi.org/10.3390/su9040618
Chicago/Turabian StyleLee, Ho-Sun, Young-Ah Jeon, Young-Yi Lee, Gi-An Lee, Sebastin Raveendar, and Kyung Ho Ma. 2017. "Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS)" Sustainability 9, no. 4: 618. https://doi.org/10.3390/su9040618