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Sustainability 2017, 9(2), 243; doi:10.3390/su9020243

Characterization of the Microbial Communities in Rumen Fluid Inoculated Reactors for the Biogas Digestion of Wheat Straw

1
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China
2
Water Affairs Research Institute, North China University of Water Resources and Electric Power, Zhengzhou 450045, China
*
Author to whom correspondence should be addressed.
Academic Editor: Emmanuel K. Ackom
Received: 17 December 2016 / Revised: 23 January 2017 / Accepted: 4 February 2017 / Published: 10 February 2017
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Abstract

The present study investigated the effect of rumen fluid (RF) concentration on the methane production through anaerobic digestion of wheat straw in batch mode, and compared the microbial communities in RF and RF inoculated reactors by 16S rRNA genes sequencing. Six levels of RF concentration including 1%, 5%, 10%, 15%, 20% and 25% (v/v) were used in reactors R1, R5, R10, R15, R20 and R25 respectively. The results revealed that lower than or equal to 5% RF concentrations resulted in reactor acidification and low methane production. The highest methane yield of 106 mL CH4 g VS−1 was achieved in R10, whereas higher RF concentrations than 10% could not improve the methane production significantly. Methanosarcina barkeri was abundant in the well-working reactors, and Methanobacterium was dominant in the poor-working reactors, implying the archaeal communities in reactors had changed greatly from the Methanobrevibacter-dominated RF. Although the relative abundance of Clostridium and Ruminococcus were greatly different between RF and reactors, the Bacteroidetes and Firmicutes communities were dominant in all the tested samples. The results indicated that the in vitro anaerobic conditions had altered the rumen methanogenic communities significantly and the facultative acetoclastic Methanosarcina was important for the methane production in the RF seeded reactors. View Full-Text
Keywords: anaerobic digestion; inoculum concentration; lignocellulose; methanogenesis; rumen fluid anaerobic digestion; inoculum concentration; lignocellulose; methanogenesis; rumen fluid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, K.; Zhu, H.; Zhang, Y.; Zhang, H. Characterization of the Microbial Communities in Rumen Fluid Inoculated Reactors for the Biogas Digestion of Wheat Straw. Sustainability 2017, 9, 243.

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