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Sustainability 2016, 8(9), 764; doi:10.3390/su8090764

Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization Methods

Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
China National Publications Import & Export (Group) Corporation, Beijing 100020, China
Amoy—BUCT Industrial Bio-technovation Institute, Amoy 361022, China
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
Author to whom correspondence should be addressed.
Academic Editor: Michael Wachendorf
Received: 2 June 2016 / Revised: 29 July 2016 / Accepted: 1 August 2016 / Published: 24 August 2016
(This article belongs to the Special Issue Biomass Energy Conversion)
View Full-Text   |   Download PDF [4317 KB, uploaded 24 August 2016]   |  


Lipase from Candida sp. 99–125 is widely employed to catalyzed transesterification and can be used for biodiesel production. In this study, the lipase was immobilized by combined adsorption and entrapment to catalyze biodiesel production from waste cooking oil (WCO) via transesterification, and investigating co-immobilizing agents as additives according to the enzyme activity. The addition of the mixed co-immobilizing agents has positive effects on the activities of the immobilized lipase. Three different immobilizing methods were compared by the conversion ratio of biodiesel and structured by Atom Force Microscopy (AFM) and Scanning Electron Microscopy (SEM), respectively. It was found that entrapment followed by adsorption was the best method. The effect of the co-immobilizing agent amount, lipase dosage, water content, and reuse ability of the immobilized lipase was investigated. By comparison with previous research, this immobilized lipase showed good reuse ability: the conversion ratio excesses 70% after 10 subsequent reactions, in particular, was better than Novozym435 and TLIM on waste cooking oil for one unit of lipase. View Full-Text
Keywords: immobilization; lipase; co-immobilizing agent; biodiesel; biocatalyst immobilization; lipase; co-immobilizing agent; biodiesel; biocatalyst

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhao, K.; Di, Q.; Cao, X.; Wang, M.; Deng, L.; Wang, F. Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization Methods. Sustainability 2016, 8, 764.

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