Pharmaceutics 2013, 5(2), 246-260; doi:10.3390/pharmaceutics5020246
Article

Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery

Received: 25 February 2013; in revised form: 2 April 2013 / Accepted: 12 April 2013 / Published: 23 April 2013
(This article belongs to the Special Issue Drug Delivery Using Nanotechnology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro.
Keywords: blood-brain barrier; endothelium; magnetofection; magnetic field; nanoparticle; transfection
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MDPI and ACS Style

Linemann, T.; Thomsen, L.B.; Jardin, K.G.; Laursen, J.C.; Jensen, J.B.; Lichota, J.; Moos, T. Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery. Pharmaceutics 2013, 5, 246-260.

AMA Style

Linemann T, Thomsen LB, Jardin KG, Laursen JC, Jensen JB, Lichota J, Moos T. Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery. Pharmaceutics. 2013; 5(2):246-260.

Chicago/Turabian Style

Linemann, Thomas; Thomsen, Louiza B.; Jardin, Kristian G.; Laursen, Jens C.; Jensen, Jesper B.; Lichota, Jacek; Moos, Torben. 2013. "Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery." Pharmaceutics 5, no. 2: 246-260.

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