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Pharmaceutics 2011, 3(1), 107-124; doi:10.3390/pharmaceutics3010107
Review

Cell Migration and Invasion Assays as Tools for Drug Discovery

*  and
Platypus Technologies, LLC, 5520 Nobel Drive, Suite 100, Madison, WI 53711, USA
* Author to whom correspondence should be addressed.
Received: 14 February 2011 / Revised: 3 March 2011 / Accepted: 10 March 2011 / Published: 11 March 2011
(This article belongs to the Special Issue Drug Discovery Tools)
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Abstract

Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays.
Keywords: cell migration; cell invasion; high throughput screening; high content analysis; phenotypic assays; drug discovery; 3-dimensional assays; wound healing; cancer metastasis cell migration; cell invasion; high throughput screening; high content analysis; phenotypic assays; drug discovery; 3-dimensional assays; wound healing; cancer metastasis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hulkower, K.I.; Herber, R.L. Cell Migration and Invasion Assays as Tools for Drug Discovery. Pharmaceutics 2011, 3, 107-124.

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