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Cell Migration and Invasion Assays as Tools for Drug Discovery
Platypus Technologies, LLC, 5520 Nobel Drive, Suite 100, Madison, WI 53711, USA
* Author to whom correspondence should be addressed.
Received: 14 February 2011; in revised form: 3 March 2011 / Accepted: 10 March 2011 / Published: 11 March 2011
Abstract: Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays.
Keywords: cell migration; cell invasion; high throughput screening; high content analysis; phenotypic assays; drug discovery; 3-dimensional assays; wound healing; cancer metastasis
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Hulkower, K.I.; Herber, R.L. Cell Migration and Invasion Assays as Tools for Drug Discovery. Pharmaceutics 2011, 3, 107-124.
Hulkower KI, Herber RL. Cell Migration and Invasion Assays as Tools for Drug Discovery. Pharmaceutics. 2011; 3(1):107-124.
Hulkower, Keren I.; Herber, Renee L. 2011. "Cell Migration and Invasion Assays as Tools for Drug Discovery." Pharmaceutics 3, no. 1: 107-124.