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Forests 2018, 9(2), 88; doi:10.3390/f9020088

Genome-Wide Analysis of Gene and microRNA Expression in Diploid and Autotetraploid Paulownia fortunei (Seem) Hemsl. under Drought Stress by Transcriptome, microRNA, and Degradome Sequencing

Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui District, 450002 Zhengzhou, China
These authors are the co-first authors (Suyan Niu and Zhenli Zhao contributed equally to this work).
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Received: 14 January 2018 / Revised: 7 February 2018 / Accepted: 9 February 2018 / Published: 13 February 2018
(This article belongs to the Special Issue Genetics and Genomics of Forest Trees)
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Abstract

Drought is a common and recurring climatic condition in many parts of the world, and it can have disastrous impacts on plant growth and development. Many genes involved in the drought response of plants have been identified. Transcriptome, microRNA (miRNA), and degradome analyses are rapid ways of identifying drought-responsive genes. The reference genome sequence of Paulownia fortunei (Seem) Hemsl. is now available, which makes it easier to explore gene expression, transcriptional regulation, and post-transcriptional in this species. In this study, four transcriptome, small RNA, and degradome libraries were sequenced by Illumina sequencing, respectively. A total of 258 genes and 11 miRNAs were identified for drought-responsive genes and miRNAs in P. fortunei. Degradome sequencing detected 28 miRNA target genes that were cleaved by members of nine conserved miRNA families and 12 novel miRNAs. The results here will contribute toward enriching our understanding of the response of Paulownia fortunei trees to drought stress and may provide new direction for further experimental studies related the development of molecular markers, the genetic map construction, and other genomic research projects in Paulownia. View Full-Text
Keywords: Paulownia fortunei; gene expression; transcriptome; microRNA; degradome; drought Paulownia fortunei; gene expression; transcriptome; microRNA; degradome; drought
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Zhao, Z.; Niu, S.; Fan, G.; Deng, M.; Wang, Y. Genome-Wide Analysis of Gene and microRNA Expression in Diploid and Autotetraploid Paulownia fortunei (Seem) Hemsl. under Drought Stress by Transcriptome, microRNA, and Degradome Sequencing. Forests 2018, 9, 88.

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