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Forests 2017, 8(1), 27; doi:10.3390/f8010027

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Universidade Federal do Paran√°, Curitiba, CEP 80035-050, PR, Brazil
Embrapa Florestas, Colombo, CEP 83411-00, PR, Brazil
Centre for Genetics, Ecology and Physiology, University of the Sunshine Coast, Maroochydore DC, QLD 4558, Australia
Author to whom correspondence should be addressed.
Academic Editors: Jarmo K. Holopainen and Timothy A. Martin
Received: 8 August 2016 / Revised: 11 January 2017 / Accepted: 12 January 2017 / Published: 17 January 2017
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Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees. View Full-Text
Keywords: adventitious rooting; auxin; cloning; cuttings; indole-3-acetic acid; indole-3-butyric acid; indolyl-3-acetic acid adventitious rooting; auxin; cloning; cuttings; indole-3-acetic acid; indole-3-butyric acid; indolyl-3-acetic acid

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Stuepp, C.A.; Wendling, I.; Trueman, S.J.; Koehler, H.S.; Zuffellato-Ribas, K.C. The Use of Auxin Quantification for Understanding Clonal Tree Propagation. Forests 2017, 8, 27.

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