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Forests 2014, 5(2), 347-362; doi:10.3390/f5020347
Article

Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine

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Received: 8 October 2013; in revised form: 15 February 2014 / Accepted: 18 February 2014 / Published: 24 February 2014
(This article belongs to the Special Issue Fusiform Rust Disease—Biology and Management of Resistance)
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Abstract: Fusiform rust resistance can involve gene-for-gene interactions where resistance (Fr) genes in the host interact with corresponding avirulence genes in the pathogen, Cronartium quercuum f.sp. fusiforme (Cqf). Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and 10 gall mixtures). We used single nucleotide polymorphism (SNP) genotypes at sufficient density to ensure linkage between segregating markers and Fr genes identifying SNPs that explained high proportions of variance in disease incidence using BayesCp, that also were significant using Bayesian Association with Missing Data (BAMD) software. Two SNPs mapped near Fr1 and generated significant LOD scores in single marker regression analyses for Fr1/fr1 parent 17 as well as four other parents. One SNP mapped near Fr8 and was significant for parent 28. Two SNPs mapped to linkage groups not previously shown to contain Fr genes and were significant for three parents. Parent 2 showed evidence of Fr gene stacking. Our results suggest that it is feasible to identify trees segregating for Fr genes, and to map Fr genes, based on parental analysis of SNPs that cosegregate with disease incidence in designed resistance screening trials.
Keywords: Pinus taeda; major gene resistance; gene-for-gene interaction; single nucleotide polymorphism (SNP); BayesCp; Bayesian Association with Missing Data (BAMD); logarithm of the odds (LOD) Pinus taeda; major gene resistance; gene-for-gene interaction; single nucleotide polymorphism (SNP); BayesCp; Bayesian Association with Missing Data (BAMD); logarithm of the odds (LOD)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Quesada, T.; Resende Jr., M.F.; Muñoz, P.; Wegrzyn, J.L.; Neale, D.B.; Kirst, M.; Peter, G.F.; Gezan, S.A.; Nelson, C.D.; Davis, J.M. Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine. Forests 2014, 5, 347-362.

AMA Style

Quesada T, Resende Jr. MF, Muñoz P, Wegrzyn JL, Neale DB, Kirst M, Peter GF, Gezan SA, Nelson CD, Davis JM. Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine. Forests. 2014; 5(2):347-362.

Chicago/Turabian Style

Quesada, Tania; Resende Jr., Marcio F.; Muñoz, Patricio; Wegrzyn, Jill L.; Neale, David B.; Kirst, Matias; Peter, Gary F.; Gezan, Salvador A.; Nelson, C. D.; Davis, John M. 2014. "Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine." Forests 5, no. 2: 347-362.


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