Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimen Preparation
2.2. Apatite Precipitate
2.3. Ion Concentration
2.4. HDPCs Isolation and Culture
2.5. COL and FN Secretion
2.6. Cell Adhesion and Proliferation Assay
2.7. Cell Morphology
2.8. Immunofluorescence Staining
2.9. Osteocalcin Formation
2.10. Statistical Analysis
3. Results
3.1. Characterization of CS in Different DMEM
3.2. Ion Concentration
3.3. COL and FN Secretion
3.4. Cell Adhesion and Proliferation
3.5. Cell Morphology
3.6. Osteocalcin Expression
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lai, W.-Y.; Chen, Y.-W.; Kao, C.-T.; Hsu, T.-T.; Huang, T.-H.; Shie, M.-Y. Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates. Materials 2015, 8, 4491-4504. https://doi.org/10.3390/ma8074491
Lai W-Y, Chen Y-W, Kao C-T, Hsu T-T, Huang T-H, Shie M-Y. Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates. Materials. 2015; 8(7):4491-4504. https://doi.org/10.3390/ma8074491
Chicago/Turabian StyleLai, Wei-Yun, Yi-Wen Chen, Chia-Tze Kao, Tuan-Ti Hsu, Tsui-Hsien Huang, and Ming-You Shie. 2015. "Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates" Materials 8, no. 7: 4491-4504. https://doi.org/10.3390/ma8074491