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Materials 2015, 8(11), 7269-7277; doi:10.3390/ma8115372

Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes

1
BK21 PLUS Project, Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea
2
Safety Research Team/Skin Research Division, Amore-Pacific R&D Center, 1920 Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Klara Hernadi
Received: 10 September 2015 / Revised: 14 October 2015 / Accepted: 20 October 2015 / Published: 29 October 2015
(This article belongs to the Section Biomaterials)
View Full-Text   |   Download PDF [1457 KB, uploaded 29 October 2015]   |  

Abstract

Dental luting cements are commonly used in dentistry for cementation of prosthetic restoration. Many previous studies focused on the measurement of the cell viability as the method of cytotoxicity evaluation during biocompatibility study for the material. In this study, the biocompatibility of various dental luting cements were evaluated using the new method of cytokine release measurement in order to better simulate inflammatory reactions in animal or clinical model using two different oral cells; immortalized human gingival fibroblast and immortalized human oral keratinocytes. Cells were exposed to extractions of various commercially available dental luting cements for different durations. Cytokines of IL-1α and IL-8 were measured from the supernatants of the cells and the results were then compared to the conventional MTT viability test. The result from the conventional cell viability study showed a relatively simple and straight forward indication that only one of the dental luting cements tested in this study was cytotoxic with increasing duration of exposure for both cells. Meanwhile, the result from the cytokine measurement study was much more complex at the time point they were measured, type of cells used for the study and the type of cytokines measured, all of which influenced the interpretation of the results. Hence, the better understanding of the cytokine release would be required for the application in biocompatibility evaluation. View Full-Text
Keywords: biocompatibility; cytokine; cytotoxicity; dental luting cement; fibroblast; inflammation; keratinocyte biocompatibility; cytokine; cytotoxicity; dental luting cement; fibroblast; inflammation; keratinocyte
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kwon, J.-S.; Piao, Y.-Z.; Cho, S.-A.; Yang, S.-Y.; Kim, J.H.; An, S.; Kim, K.-M. Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes. Materials 2015, 8, 7269-7277.

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