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Materials 2013, 6(11), 4911-4929; doi:10.3390/ma6114911
Article

Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

1,2
, 1,3,*  and 4
1 Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan 2 Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan 3 Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kweishan, Taoyuan 333, Taiwan 4 Department of Mechanical Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
* Author to whom correspondence should be addressed.
Received: 7 August 2013 / Revised: 9 October 2013 / Accepted: 18 October 2013 / Published: 25 October 2013
Download PDF [648 KB, 29 October 2013; original version 25 October 2013]

Abstract

Three-dimensional porous polycaprolactone (PCL) scaffolds with consistent inter-pore channels, 83% porosity and 300–400 μm pore size were fabricated via selective laser sintering. The PCL scaffold was combined with platelet-rich plasma (PRP) to form a bioactive composite and studied for potential application in bone tissue engineering using porcine adipose-derived stem cells (PASCs). The PCL/PRP/PASCs construct showed enhanced cell seeding efficiency and synergistically increased the differentiation capability of PASCs in osteogenic medium toward the osteoblast lineage, judging from elevated alkaline phosphatase activity and up-regulated osteogenic genes expression. For in vivo study, a 3 cm × 3 cm mandible defect was created in pigs and reconstructed by implanting acellular PCL scaffolds or PCL/PRP/PASCs constructs. Both groups showed new bone formation, however, the new bone volume was 5.1 times higher for PCL/PRP/PASCs 6 months post-operation. The bone density was less and loose in the acellular PCL group and the Young’s modulus was only 29% of normal bone. In contrast, continued and compact bone formation was found in PCL/PRP/PASCs and the Young’s modulus was 81% that of normal bone. Masson’s trichrome stain, immunohistochemical analysis of osteocalcin and collagen type I also confirmed new bone formation.
Keywords: bone tissue engineering; selective laser sintering; polycaprolactone; platelet-rich plasma; adipose-derived stem cells bone tissue engineering; selective laser sintering; polycaprolactone; platelet-rich plasma; adipose-derived stem cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liao, H.-T.; Chen, J.-P.; Lee, M.-Y. Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma. Materials 2013, 6, 4911-4929.

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