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Materials 2013, 6(11), 4911-4929; doi:10.3390/ma6114911
Article

Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

1,2
,
1,3,*  and 4
1 Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan 2 Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Craniofacial Research Center, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan 3 Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kweishan, Taoyuan 333, Taiwan 4 Department of Mechanical Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
* Author to whom correspondence should be addressed.
Received: 7 August 2013 / Revised: 9 October 2013 / Accepted: 18 October 2013 / Published: 25 October 2013
(This article belongs to the Section Biomaterials)
View Full-Text   |   Download PDF [648 KB, 29 October 2013; original version 25 October 2013]   |  

Abstract

Three-dimensional porous polycaprolactone (PCL) scaffolds with consistent inter-pore channels, 83% porosity and 300–400 μm pore size were fabricated via selective laser sintering. The PCL scaffold was combined with platelet-rich plasma (PRP) to form a bioactive composite and studied for potential application in bone tissue engineering using porcine adipose-derived stem cells (PASCs). The PCL/PRP/PASCs construct showed enhanced cell seeding efficiency and synergistically increased the differentiation capability of PASCs in osteogenic medium toward the osteoblast lineage, judging from elevated alkaline phosphatase activity and up-regulated osteogenic genes expression. For in vivo study, a 3 cm × 3 cm mandible defect was created in pigs and reconstructed by implanting acellular PCL scaffolds or PCL/PRP/PASCs constructs. Both groups showed new bone formation, however, the new bone volume was 5.1 times higher for PCL/PRP/PASCs 6 months post-operation. The bone density was less and loose in the acellular PCL group and the Young’s modulus was only 29% of normal bone. In contrast, continued and compact bone formation was found in PCL/PRP/PASCs and the Young’s modulus was 81% that of normal bone. Masson’s trichrome stain, immunohistochemical analysis of osteocalcin and collagen type I also confirmed new bone formation.
Keywords: bone tissue engineering; selective laser sintering; polycaprolactone; platelet-rich plasma; adipose-derived stem cells bone tissue engineering; selective laser sintering; polycaprolactone; platelet-rich plasma; adipose-derived stem cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Liao, H.-T.; Chen, J.-P.; Lee, M.-Y. Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma. Materials 2013, 6, 4911-4929.

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