Adsorption of Milk Proteins (β-Casein and β-Lactoglobulin) and BSA onto Hydrophobic Surfaces
Biocolloid and Fluid Physics Group, Department of Applied Physics, University of Granada, Av. Fuentenueva 2, E-18001 Granada, Spain
CNRS, Centre de Recherche Paul Pascal (CRPP), UPR 8641, F3300 Pessac, France
Université de Bordeaux, CRPP, UPR 8641, F-33600 Pessac, France
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, E-08193 Bellaterra, Barcelona, Spain
Author to whom correspondence should be addressed.
Received: 1 June 2017 / Revised: 30 June 2017 / Accepted: 24 July 2017 / Published: 2 August 2017
Here, we study films of proteins over planar surfaces and protein-coated microspheres obtained from the adsorption of three different proteins (
-lactoglobulin and bovine serum albumin (BSA)). The investigation of protein films in planar surfaces is performed by combining quartz crystal microbalance (QCM) and atomic force microscopy (AFM) measurements with all-atomic molecular dynamics (MD) simulations. We found that BSA and
-lactoglobulin form compact monolayers, almost without interstices between the proteins. However,
-casein adsorbs forming multilayers. The study of the electrokinetic mobility of protein-coated latex microspheres shows substantial condensation of ions from the buffer over the complexes, as predicted from ion condensation theories. The electrokinetic behavior of the latex-protein complexes is dominated by the charge of the proteins and the phenomenon of ion condensation, whereas the charge of the latex colloids plays only a minor role.
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Pérez-Fuentes, L.; Drummond, C.; Faraudo, J.; Bastos-González, D. Adsorption of Milk Proteins (β-Casein and β-Lactoglobulin) and BSA onto Hydrophobic Surfaces. Materials 2017, 10, 893.
Pérez-Fuentes L, Drummond C, Faraudo J, Bastos-González D. Adsorption of Milk Proteins (β-Casein and β-Lactoglobulin) and BSA onto Hydrophobic Surfaces. Materials. 2017; 10(8):893.
Pérez-Fuentes, Leonor; Drummond, Carlos; Faraudo, Jordi; Bastos-González, Delfi. 2017. "Adsorption of Milk Proteins (β-Casein and β-Lactoglobulin) and BSA onto Hydrophobic Surfaces." Materials 10, no. 8: 893.
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