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Materials 2017, 10(8), 893; doi:10.3390/ma10080893

Adsorption of Milk Proteins (β-Casein and β-Lactoglobulin) and BSA onto Hydrophobic Surfaces

1
Biocolloid and Fluid Physics Group, Department of Applied Physics, University of Granada, Av. Fuentenueva 2, E-18001 Granada, Spain
2
CNRS, Centre de Recherche Paul Pascal (CRPP), UPR 8641, F3300 Pessac, France
3
Université de Bordeaux, CRPP, UPR 8641, F-33600 Pessac, France
4
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, E-08193 Bellaterra, Barcelona, Spain
*
Author to whom correspondence should be addressed.
Received: 1 June 2017 / Revised: 30 June 2017 / Accepted: 24 July 2017 / Published: 2 August 2017
(This article belongs to the Section Structure Analysis and Characterization)
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Abstract

Here, we study films of proteins over planar surfaces and protein-coated microspheres obtained from the adsorption of three different proteins ( β -casein, β -lactoglobulin and bovine serum albumin (BSA)). The investigation of protein films in planar surfaces is performed by combining quartz crystal microbalance (QCM) and atomic force microscopy (AFM) measurements with all-atomic molecular dynamics (MD) simulations. We found that BSA and β -lactoglobulin form compact monolayers, almost without interstices between the proteins. However, β -casein adsorbs forming multilayers. The study of the electrokinetic mobility of protein-coated latex microspheres shows substantial condensation of ions from the buffer over the complexes, as predicted from ion condensation theories. The electrokinetic behavior of the latex-protein complexes is dominated by the charge of the proteins and the phenomenon of ion condensation, whereas the charge of the latex colloids plays only a minor role. View Full-Text
Keywords: proteins; QCM; MD simulations; hydrophobic effect; ion condensation; electrokinetic mobility proteins; QCM; MD simulations; hydrophobic effect; ion condensation; electrokinetic mobility
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pérez-Fuentes, L.; Drummond, C.; Faraudo, J.; Bastos-González, D. Adsorption of Milk Proteins (β-Casein and β-Lactoglobulin) and BSA onto Hydrophobic Surfaces. Materials 2017, 10, 893.

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