Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi
AbstractA starch-based ethanol facility producing 200,000 m3 ethanol/year also produces ca. 2 million m3 thin stillage, which can be used to improve the entire process. In this work, five food-grade filamentous fungi, including a Zygomycete and four Ascomycetes were successfully grown in thin stillage containing 9% solids. Cultivation with Neurospora intermedia led to the production of ca. 16 g·L−1 biomass containing 56% (w/w) crude protein, a reduction of 34% of the total solids, and 5 g·L−1 additional ethanol. In an industrial ethanol production process (200,000 m3 ethanol/year), this can potentially lead to the production of 11,000 m3 extra ethanol per year. Cultivation with Aspergillus oryzae resulted in 19 g·L−1 biomass containing 48% (w/w) crude protein and the highest reduction of the thin stillage glycerol (54%) among the Ascomycetes. Cultivation with Rhizopus sp. produced up to 15 g·L−1 biomass containing 55% (w/w) crude protein. The spent thin stillage had been reduced up to 85%, 68% and 21% regarding lactic acid, glycerol and total solids, respectively. Therefore, N. intermedia, in particular, has a high potential to improve the ethanol process via production of additional ethanol and high-quality biomass, which can be considered for animal feed applications such as for fish feed.
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Ferreira, J.A.; Lennartsson, P.R.; Taherzadeh, M.J. Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi. Energies 2014, 7, 3872-3885.
Ferreira JA, Lennartsson PR, Taherzadeh MJ. Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi. Energies. 2014; 7(6):3872-3885.Chicago/Turabian Style
Ferreira, Jorge A.; Lennartsson, Patrik R.; Taherzadeh, Mohammad J. 2014. "Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi." Energies 7, no. 6: 3872-3885.