Energies 2014, 7(6), 3840-3857; doi:10.3390/en7063840
Article

Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity

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Received: 10 March 2014; in revised form: 7 May 2014 / Accepted: 11 June 2014 / Published: 19 June 2014
(This article belongs to the Special Issue Renewable Energy for Agriculture)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The aim of this study was to induce lipid accumulation in Chlorella cells by creating stressful growth conditions. Chlorella vulgaris CCALA 896 was grown under various batch growth modes in basal and modified BG-11 and Kolkwitz culture broths, using a continuous light regimen of 150 µE/m2/s, at 30 °C. In order to perform the experiments, two indoor photobioreactor shapes were used: a cylindrical glass photobioreactor (CGPBR) with a working volume of 350 mL, and a flat glass photobioreactor (FGPBR) with a working volume of 550 mL. Stress-eliciting conditions, such as nitrogen and phosphorous starvation, were imposed in order to induce lipid accumulation. The results demonstrated that more than 56% of the lipids can be accumulated in Chlorella biomass grown under two-phase batch growth conditions. The highest biomass productivity of 0.30 g/L/d was obtained at the highest nominal dilution rate (0.167 day−1) during a semi-continuous regimen, using a modified Kolkwitz medium. During the pH-stress cycles, the amount of lipids did not increase significantly and a flocculation of Chlorella cells was noted.
Keywords: Chlorella vulgaris; indoor photobioreactors; nitrogen/phosphorus starvation; biomass productivity; oil-rich biomass
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MDPI and ACS Style

Vaičiulytė, S.; Padovani, G.; Kostkevičienė, J.; Carlozzi, P. Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity. Energies 2014, 7, 3840-3857.

AMA Style

Vaičiulytė S, Padovani G, Kostkevičienė J, Carlozzi P. Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity. Energies. 2014; 7(6):3840-3857.

Chicago/Turabian Style

Vaičiulytė, Sigita; Padovani, Giulia; Kostkevičienė, Jolanta; Carlozzi, Pietro. 2014. "Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity." Energies 7, no. 6: 3840-3857.

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