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Energies 2013, 6(4), 2175-2190; doi:10.3390/en6042175

Bio-Hydrogen Production from Pineapple Waste Extract by Anaerobic Mixed Cultures

1,2,*  and 3
1 Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand 2 Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand 3 Department of Agriculture, Faculty of Food and Agricultural Technology, Phibulsongkram Rajabhat University, Phitsanulok 65000, Thailand
* Author to whom correspondence should be addressed.
Received: 27 December 2012 / Revised: 7 March 2013 / Accepted: 2 April 2013 / Published: 18 April 2013
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A statistical experimental design was employed to optimize factors that affect the production of hydrogen from the glucose contained in pineapple waste extract by anaerobic mixed cultures. Results from Plackett-Burman design indicated that substrate concentration, initial pH and FeSO4 concentration had a statistically significant (p ≤ 0.05) influence on the hydrogen production potential (Ps) and the specific hydrogen production rate (SHPR). The path of steepest ascent was undertaken to approach the optimal region of these three significant factors which was then optimized using response surface methodology (RSM) with central composite design (CCD). The presence of a substrate concentration of 25.76 g-total sugar/L, initial pH of 5.56, and FeSO4 concentration of 0.81 g/L gave a maximum predicted Ps of 5489 mL H2/L, hydrogen yield of 1.83 mol H2/mol glucose, and SHPR of 77.31 mL H2/g-volatile suspended solid (VSS) h. A verification experiment indicated highly reproducible results with the observed Ps and SHPR being only 1.13% and 1.14% different from the predicted values.
Keywords: pineapple waste extract; bio-hydrogen pineapple waste extract; bio-hydrogen
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Reungsang, A.; Sreela-or, C. Bio-Hydrogen Production from Pineapple Waste Extract by Anaerobic Mixed Cultures. Energies 2013, 6, 2175-2190.

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