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Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus
Department of Bioengineering, Tatung University, 40 Chungshan N. Rd. Sec. 3, Taipei 10452, Taiwan
* Author to whom correspondence should be addressed.
Received: 8 October 2012; in revised form: 20 December 2012 / Accepted: 8 March 2013 / Published: 21 March 2013
Abstract: Carrot pomace, a major agricultural waste from the juice industry, was used as a feedstock for bioethanol production by fermentation with the thermotolerant yeast Kluyveromyces marxianus. Treatment of the carrot pomace with AccelleraseTM 1000 and pectinase at 50 °C for 84 h, resulted in conversion of 42% of its mass to fermentable sugars, mainly glucose, fructose, and sucrose. Simultaneous saccharification and fermentation (SSF) at 42 °C was performed on 10% (w/v) carrot pomace; the concentration of ethanol reached 18 g/L and the yield of ethanol from carrot pomace was 0.18 g/g. The highest ethanol concentration of 37 g/L was observed with an additional charge of 10% supplemented to the original 10% of carrot pomace after 12 h; the corresponding yield was 0.185 g/g. Our results clearly demonstrated the potential of combining a SSF process with thermotolerant yeast for the production of bioethanol using carrot pomace as a feedstock.
Keywords: bioethanol; carrot pomace; Kluyveromyces marxianus; simultaneous saccharification and fermentation
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MDPI and ACS Style
Yu, C.-Y.; Jiang, B.-H.; Duan, K.-J. Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus. Energies 2013, 6, 1794-1801.
Yu C-Y, Jiang B-H, Duan K-J. Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus. Energies. 2013; 6(3):1794-1801.
Yu, Chi-Yang; Jiang, Bo-Hong; Duan, Kow-Jen. 2013. "Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus." Energies 6, no. 3: 1794-1801.