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Energies 2017, 10(1), 35; doi:10.3390/en10010035

Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

1
Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
2
Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia
3
Department of Mechanical Engineering, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia
4
Department of Mechanical Engineering, Faculty of Engineering, The University of Melbourne, Parkville, VIC 3010, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas E. Amidon
Received: 14 June 2016 / Revised: 30 August 2016 / Accepted: 4 September 2016 / Published: 1 January 2017
(This article belongs to the Collection Bioenergy and Biofuel)
View Full-Text   |   Download PDF [2053 KB, uploaded 1 January 2017]   |  

Abstract

Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. View Full-Text
Keywords: Manihot glaziovii (M. glaziovii); optimization; hydrolysis; fermentation; bioethanol; alternative fuel Manihot glaziovii (M. glaziovii); optimization; hydrolysis; fermentation; bioethanol; alternative fuel
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Sebayang, A.H.; Hassan, M.H.; Ong, H.C.; Dharma, S.; Silitonga, A.S.; Kusumo, F.; Mahlia, T.M.I.; Bahar, A.H. Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology. Energies 2017, 10, 35.

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