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Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells
Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, Ilan 262, Taiwan
* Author to whom correspondence should be addressed.
Received: 9 September 2011; in revised form: 8 October 2011 / Accepted: 12 October 2011 / Published: 19 October 2011
Abstract: Pardaxin is an antimicrobial peptide (AMP) that was first isolated from secretions of the Red Sea Moses sole. The role of pardaxin in inducing apoptosis for preventing cancer has not yet been investigated. In the present study, we examined the antitumor activity of pardaxin against human fibrosarcoma HT-1080 cells; pardaxin inhibited cell proliferation by inducing apoptosis, as demonstrated by an increase in the externalization of plasma membrane phosphatidylserine and the presence of chromatin condensation. Additionally, pardaxin-treated cells showed elevation of caspase-3/7 activities, disruption of the mitochondrial membrane potential, and accumulation of reactive oxygen species (ROS) production. Inhibition of ROS production and caspase-3/7 activities reduced pardaxin-induced effects. Taken together, these findings suggest that pardaxin may be a potential anticancer agent for selectively inducing apoptosis in cancer cells.
Keywords: antimicrobial peptide; pardaxin; apoptosis
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MDPI and ACS Style
Huang, T.-C.; Lee, J.-F.; Chen, J.-Y. Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells. Mar. Drugs 2011, 9, 1995-2009.
Huang T-C, Lee J-F, Chen J-Y. Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells. Marine Drugs. 2011; 9(10):1995-2009.
Huang, Tsui-Chin; Lee, Jheng-Fong; Chen, Jyh-Yih. 2011. "Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells." Mar. Drugs 9, no. 10: 1995-2009.