Next Article in Journal
Previous Article in Journal
Mar. Drugs 2011, 9(10), 1995-2009; doi:10.3390/md9101995
Article

Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells

,
 and *
Received: 9 September 2011; in revised form: 8 October 2011 / Accepted: 12 October 2011 / Published: 19 October 2011
View Full-Text   |   Download PDF [1704 KB, uploaded 19 October 2011]
Abstract: Pardaxin is an antimicrobial peptide (AMP) that was first isolated from secretions of the Red Sea Moses sole. The role of pardaxin in inducing apoptosis for preventing cancer has not yet been investigated. In the present study, we examined the antitumor activity of pardaxin against human fibrosarcoma HT-1080 cells; pardaxin inhibited cell proliferation by inducing apoptosis, as demonstrated by an increase in the externalization of plasma membrane phosphatidylserine and the presence of chromatin condensation. Additionally, pardaxin-treated cells showed elevation of caspase-3/7 activities, disruption of the mitochondrial membrane potential, and accumulation of reactive oxygen species (ROS) production. Inhibition of ROS production and caspase-3/7 activities reduced pardaxin-induced effects. Taken together, these findings suggest that pardaxin may be a potential anticancer agent for selectively inducing apoptosis in cancer cells.
Keywords: antimicrobial peptide; pardaxin; apoptosis antimicrobial peptide; pardaxin; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Huang, T.-C.; Lee, J.-F.; Chen, J.-Y. Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells. Mar. Drugs 2011, 9, 1995-2009.

AMA Style

Huang T-C, Lee J-F, Chen J-Y. Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells. Marine Drugs. 2011; 9(10):1995-2009.

Chicago/Turabian Style

Huang, Tsui-Chin; Lee, Jheng-Fong; Chen, Jyh-Yih. 2011. "Pardaxin, an Antimicrobial Peptide, Triggers Caspase-Dependent and ROS-Mediated Apoptosis in HT-1080 Cells." Mar. Drugs 9, no. 10: 1995-2009.



Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert