Abstract: Neurotoxicity induced in fish by domoic acid (DA) was assessed with respect to occurrence of neurotoxic signs, lethality, and histopathology by light microscopy. Sparus aurata were exposed to a single dose of DA by intraperitoneal (i.p.) injection of 0, 0.45, 0.9, and 9.0 mg DA kg−1 bw. Mortality (66.67 ± 16.67%) was only observed in dose of 9.0 mg kg−1 bw. Signs of neurological toxicity were detected for the doses of 0.9 and 9.0 mg DA kg−1 bw. Furthermore, the mean concentrations (±SD) of DA detected by HPLC-UV in extracts of brain after exposure to 9.0 mg DA kg−1 bw were 0.61 ± 0.01, 0.96 ± 0.00, and 0.36 ± 0.01 mg DA kg−1 tissue at 1, 2, and 4 hours. The lack of major permanent brain damage in S. aurata, and reversibility of neurotoxic signs, suggest that lower susceptibility to DA or neuronal recovery occurs in affected individuals.
Keywords: domoic acid; amnesic shellfish poisoning; Pseudo-nitzschia; algal neurotoxin; Sparus aurata
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Nogueira, I.; Lobo-da-Cunha, A.; Afonso, A.; Rivera, S.; Azevedo, J.; Monteiro, R.; Cervantes, R.; Gago-Martinez, A.; Vasconcelos, V. Toxic Effects of Domoic Acid in the Seabream Sparus aurata. Mar. Drugs 2010, 8, 2721-2732.
Nogueira I, Lobo-da-Cunha A, Afonso A, Rivera S, Azevedo J, Monteiro R, Cervantes R, Gago-Martinez A, Vasconcelos V. Toxic Effects of Domoic Acid in the Seabream Sparus aurata. Marine Drugs. 2010; 8(10):2721-2732.
Nogueira, Isabel; Lobo-da-Cunha, Alexandre; Afonso, António; Rivera, Socorro; Azevedo, Joana; Monteiro, Rogério; Cervantes, Rosa; Gago-Martinez, Ana; Vasconcelos, Vítor. 2010. "Toxic Effects of Domoic Acid in the Seabream Sparus aurata." Mar. Drugs 8, no. 10: 2721-2732.