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Mar. Drugs 2018, 16(8), 258; https://doi.org/10.3390/md16080258

Biochemical Characterization and Degradation Pattern of a Novel Endo-Type Bifunctional Alginate Lyase AlyA from Marine Bacterium Isoptericola halotolerans

1
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, Jiangsu, China
2
College of Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China
3
Technology Transfer Center, Nanjing Forest University, Nanjing 210037, Jiangsu, China
*
Authors to whom correspondence should be addressed.
Received: 28 June 2018 / Revised: 21 July 2018 / Accepted: 27 July 2018 / Published: 31 July 2018
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Abstract

Alginate lyases are important tools to prepare oligosaccharides with various physiological activities by degrading alginate. Particularly, the bifunctional alginate lyase can efficiently hydrolyze the polysaccharide into oligosaccharides. Herein, we cloned and identified a novel bifunctional alginate lyase, AlyA, with a high activity and broad substrate specificity from bacterium Isoptericola halotolerans NJ-05 for oligosaccharides preparation. For further applications in industry, the enzyme has been characterized and its action mode has been also elucidated. It exhibited the highest activity (7984.82 U/mg) at pH 7.5 and 55 °C. Additionally, it possessed a broad substrate specificity, showing high activities towards not only polyM (polyβ-d-mannuronate) (7658.63 U/mg), but also polyG (poly α-l-guluronate) (8643.29 U/mg). Furthermore, the Km value of AlyA towards polyG (3.2 mM) was lower than that towards sodium alginate (5.6 mM) and polyM (6.7 mM). TLC (Thin Layer Chromatography) and ESI-MS (Electrospray Ionization Mass Spectrometry) were used to study the action mode of the enzyme, showing that it can hydrolyze the substrates in an endolytic manner to release a series of oligosaccharides such as disaccharide, trisaccharide, and tetrasaccharide. This study provided extended insights into the substrate recognition and degrading pattern of the alginate lyases, with a broad substrate specificity. View Full-Text
Keywords: Isoptericola halotolerans; bifunctional alginate lyase; oligosaccharides Isoptericola halotolerans; bifunctional alginate lyase; oligosaccharides
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Zhu, B.; Ning, L.; Jiang, Y.; Ge, L. Biochemical Characterization and Degradation Pattern of a Novel Endo-Type Bifunctional Alginate Lyase AlyA from Marine Bacterium Isoptericola halotolerans. Mar. Drugs 2018, 16, 258.

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