Next Article in Journal
Neuronal Nicotinic Acetylcholine Receptor Modulators from Cone Snails
Previous Article in Journal
Smenamide A Analogues. Synthesis and Biological Activity on Multiple Myeloma Cells
Previous Article in Special Issue
Proteomic Analyses of the Unexplored Sea Anemone Bunodactis verrucosa
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Mar. Drugs 2018, 16(6), 207; https://doi.org/10.3390/md16060207

The Holo-Transcriptome of the Zoantharian Protopalythoa variabilis (Cnidaria: Anthozoa): A Plentiful Source of Enzymes for Potential Application in Green Chemistry, Industrial and Pharmaceutical Biotechnology

1
Northeast Biotechnology Network (RENORBIO), Post-Graduation Program in Biotechnology, Federal University of Ceará, Fortaleza 60440-900, Brazil
2
Laboratory of Biochemistry and Biotechnology, Institute for Marine Sciences, Federal University of Ceará, Fortaleza 60165-081, Brazil
3
State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau 519020, China
4
Department of Biology, Federal Rural University of Pernambuco, Recife 52171-900, Brazil
5
Academic Center in Vitória, Federal University of Pernambuco, Vitória de Santo Antão 50670-901, Pernambuco, Brazil
6
Laboratory of Genetics, Butantan Institute, São Paulo 05503-900, Brazil
*
Authors to whom correspondence should be addressed.
Received: 1 May 2018 / Revised: 5 June 2018 / Accepted: 8 June 2018 / Published: 13 June 2018
View Full-Text   |   Download PDF [2383 KB, uploaded 13 June 2018]   |  

Abstract

Marine invertebrates, such as sponges, tunicates and cnidarians (zoantharians and scleractinian corals), form functional assemblages, known as holobionts, with numerous microbes. This type of species-specific symbiotic association can be a repository of myriad valuable low molecular weight organic compounds, bioactive peptides and enzymes. The zoantharian Protopalythoa variabilis (Cnidaria: Anthozoa) is one such example of a marine holobiont that inhabits the coastal reefs of the tropical Atlantic coast and is an interesting source of secondary metabolites and biologically active polypeptides. In the present study, we analyzed the entire holo-transcriptome of P. variabilis, looking for enzyme precursors expressed in the zoantharian-microbiota assemblage that are potentially useful as industrial biocatalysts and biopharmaceuticals. In addition to hundreds of predicted enzymes that fit into the classes of hydrolases, oxidoreductases and transferases that were found, novel enzyme precursors with multiple activities in single structures and enzymes with incomplete Enzyme Commission numbers were revealed. Our results indicated the predictive expression of thirteen multifunctional enzymes and 694 enzyme sequences with partially characterized activities, distributed in 23 sub-subclasses. These predicted enzyme structures and activities can prospectively be harnessed for applications in diverse areas of industrial and pharmaceutical biotechnology. View Full-Text
Keywords: Zoanthidea; holo-transcriptome; cnidarian transcriptome; marine enzyme; marine biocatalyst; marine biotechnology; pharmaceutical biotechnology Zoanthidea; holo-transcriptome; cnidarian transcriptome; marine enzyme; marine biocatalyst; marine biotechnology; pharmaceutical biotechnology
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

R. L. Morlighem, J.-É.; Huang, C.; Liao, Q.; Braga Gomes, P.; Daniel Pérez, C.; de Brandão Prieto-da-Silva, Á.R.; Ming-Yuen Lee, S.; Rádis-Baptista, G. The Holo-Transcriptome of the Zoantharian Protopalythoa variabilis (Cnidaria: Anthozoa): A Plentiful Source of Enzymes for Potential Application in Green Chemistry, Industrial and Pharmaceutical Biotechnology. Mar. Drugs 2018, 16, 207.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top