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Mar. Drugs 2017, 15(5), 140; doi:10.3390/md15050140

Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells

Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
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Academic Editors: Paul Long, Orazio Taglialatela-Scafati and Alejandro M. Mayer
Received: 16 March 2017 / Revised: 24 April 2017 / Accepted: 4 May 2017 / Published: 18 May 2017
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Abstract

In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 (g/mL); an extraction temperature of 72 °C; and an extraction time of 3 h. Then, a purified polysaccharide named Cereus sinensis polysaccharide-1 (CSP-1) was obtained from the crude polysaccharide by the Diethylaminoethyl cellulose-52 (DEAE-52) cellulose chromatography column and Sephadex G-100 column. The molecular weight and monosaccharide composition of CSP-1 was determined through Gel Permeation Chromatography (GPC) and Gas Chromatography–Mass Spectrometer (GS–MS), respectively. The results showed that CSP-1 with an average molecular weight of 56,335 Da was composed of l-(−)-Fucose, d-(+)-Mannose, d-Glucose and mainly possessed 1→2, 1→2, 6, 1→4, and 1→4, 6 of glycosyl linkages. The immunomodulatory activities of CSP-1 were also evaluated using lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. The results demonstrated that CSP-1 dose-dependently protected against LPS-induced toxicity, and CSP-1 significantly inhibited the Toll-like receptor 4 (TLR-4) mRNA, myeloid differentiation factor 88 (MyD88) mRNA and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA expression of the LPS-induced THP-1 cells, as well as suppressing reactive oxygen species (ROS) generation. View Full-Text
Keywords: Cereus sinensis; polysaccharide; THP-1 cells; immunomodulatory property Cereus sinensis; polysaccharide; THP-1 cells; immunomodulatory property
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MDPI and ACS Style

Wu, J.; Zhou, X.; Zhang, M.; Yao, Y.; Han, J.; Liu, K. Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells. Mar. Drugs 2017, 15, 140.

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