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Mar. Drugs 2016, 14(3), 47; doi:10.3390/md14030047

Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi

1
School of Pharmacy, Tianjin Medical University, Tianjin 300070, China
2
Department of Pharmacolgoy, Capital Medical University, 10 Xitoutiao, Youanmen St., Beijing 100069, China
3
Linqu County Haifushan Hospital, Shandong 262600, China
*
Author to whom correspondence should be addressed.
Academic Editor: Keith B. Glaser
Received: 25 December 2015 / Revised: 26 February 2016 / Accepted: 29 February 2016 / Published: 21 March 2016
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Abstract

CS5931 is a novel anticancer agent isolated from the sea squirt Ciona savignyi. However, its content in the species is very low, and developing a novel approach for production of the polypeptide is promising. In the present study, we expressed and purified the polypeptide from E. coli, and the fermentation conditions were studied using response surface methodology. The yield of CS5931 was increased from 2.0 to 7.5 mg/L. The denaturing and renaturation conditions were also studied. Using the optimized renaturation condition, the anticancer activity of refolding CS5931 was increased significantly; the value of IC50 was decreased from 23.2 to 11.6 μM. In vivo study using xenograft nude mice bearing HCT116 cancer cells revealed that CS5931 was able to inhibit the growth of tumor significantly. The study provides a useful approach for obtaining enough amount of CS5931 for further study. This study is also important for developing the polypeptide as a novel anticancer agent. View Full-Text
Keywords: CS5931; protein expression; renaturation; antitumor activity CS5931; protein expression; renaturation; antitumor activity
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MDPI and ACS Style

Chen, X.; Xu, H.; Li, B.; Wang, F.; Chen, X.; Kong, D.; Lin, X. Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi. Mar. Drugs 2016, 14, 47.

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