Next Article in Journal
Marine Peptides: Bioactivities and Applications
Next Article in Special Issue
Investigation of Marine-Derived Fungal Diversity and Their Exploitable Biological Activities
Previous Article in Journal / Special Issue
Can Some Marine-Derived Fungal Metabolites Become Actual Anticancer Agents?
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2015, 13(7), 3992-4005; doi:10.3390/md13073992

Production of Calcaride A by Calcarisporium sp. in Shaken Flasks and Stirred Bioreactors

VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland
*
Author to whom correspondence should be addressed.
Academic Editor: Johannes F. Imhoff
Received: 30 March 2015 / Revised: 19 May 2015 / Accepted: 15 June 2015 / Published: 24 June 2015
(This article belongs to the Special Issue Bioactive Compounds from Marine Fungi)
View Full-Text   |   Download PDF [1329 KB, uploaded 24 June 2015]   |  

Abstract

Increased interest in marine resources has led to increased screening of marine fungi for novel bioactive compounds and considerable effort is being invested in discovering these metabolites. For compound discovery, small-scale cultures are adequate, but agitated bioreactors are desirable for larger-scale production. Calcarisporium sp. KF525 has recently been described to produce calcaride A, a cyclic polyester with antibiotic activity, in agitated flasks. Here, we describe improvements in the production of calcaride A in both flasks (13-fold improvement) and stirred bioreactors (200-fold improvement). Production of calcaride A in bioreactors was initially substantially lower than in shaken flasks. The cultivation pH (reduced from 6.8 to <5.4), carbon source (sucrose replacing glucose), C/N ratio and nature of mycelial growth (pellets or filaments) were important in improving calcaride A production. Up to 4.5 mg·g−1 biomass (85 mg·L−1) calcaride A were produced in the bioreactor, which was only slightly less than in shaken flasks (14 mg·g−1, 100 mg·L−1). The results demonstrate that a scalable process for calcaride A production could be developed using an iterative approach with flasks and bioreactors. View Full-Text
Keywords: marine fungi; Calcarisporium; calcaride A; stirred tank bioreactor; pH; macrocylic polyester marine fungi; Calcarisporium; calcaride A; stirred tank bioreactor; pH; macrocylic polyester
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tamminen, A.; Wang, Y.; Wiebe, M.G. Production of Calcaride A by Calcarisporium sp. in Shaken Flasks and Stirred Bioreactors. Mar. Drugs 2015, 13, 3992-4005.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top