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Mar. Drugs 2015, 13(4), 2183-2195; doi:10.3390/md13042183

Functional Recombinants Designed from a Fetuin/Asialofetuin-Specific Marine Algal Lectin, Rhodobindin

1
Department of Biology, Kongju National University, Kongju 314-701, Korea
2
Marine Biodiversity Institute of Korea, Seocheon 427-100, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Orazio Taglialatela-Scafati
Received: 10 February 2015 / Revised: 24 March 2015 / Accepted: 27 March 2015 / Published: 13 April 2015
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Abstract

Plant lectins have attracted much attention for biomedical applications including targeted drug delivery system and therapy against tumors and microbial infections. The main problem of using lectins as a biomedical tool is a batch-to-batch variation in isoforms content. The production of lectins using recombination tools has the advantage of obtaining high amounts of proteins with more precise properties, but there are only a handful of functional recombinant lectins presently available. A fetuin/asialo-fetuin specific lectin, Rhodobindin, has unique tandem repeats structure which makes it useful in exploiting for recombinant lectin. We developed three functional recombinant lectins using E. coli expression system: one from full cDNA sequence and two from fragmentary sequences of Rhodobindin. Hemagglutinating activity and solubility of the recombinant lectins were highest at OD 0.7 cell concentration at 20 °C. The optimized process developed in this study was suitable for the quality-controlled production of high amounts of soluble recombinant lectins. View Full-Text
Keywords: lectin; Rhodobindin; recombination; mass production; E. coli expression system lectin; Rhodobindin; recombination; mass production; E. coli expression system
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MDPI and ACS Style

Han, J.W.; Jung, M.G.; Shim, E.Y.; Shim, J.B.; Kim, Y.M.; Kim, G.H. Functional Recombinants Designed from a Fetuin/Asialofetuin-Specific Marine Algal Lectin, Rhodobindin. Mar. Drugs 2015, 13, 2183-2195.

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