Mar. Drugs 2014, 12(2), 1148-1156; doi:10.3390/md12021148

Article
Krempfielins N–P, New Anti-Inflammatory Eunicellins from a Taiwanese Soft Coral Cladiella krempfi
Yan-Ning Lee 1, Chi-Jen Tai 1, Tsong-Long Hwang 2 and Jyh-Horng Sheu 1,3,4,5,*
1
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; E-Mails: jennyyanningl@yahoo.com.tw (Y.-N.L.); jean801023@hotmail.com (C.-J.T.)
2
Graduate Institute of Natural Products, Chang Gung University, Taoyuan 33302, Taiwan; E-Mail: htl@mail.cgu.edu.tw
3
Division of Marine Biotechnology, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
4
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan
5
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
*
Author to whom correspondence should be addressed; E-Mail: sheu@mail.nsysu.edu.tw; Tel.: +886-7-5252000 (ext. 5030); Fax: +886-7-5255020.
Received: 11 December 2013; in revised form: 22 January 2014 / Accepted: 7 February 2014 /
Published: 21 February 2014

Abstract

: Three new eunicellin-type diterpenoids, krempfielins N–P (13), were isolated from a Taiwanese soft coral Cladiella krempfi. The structures of the new metabolites were elucidated by extensive spectroscopic analysis and by comparison with spectroscopic data of related known compounds. Compound 3 exhibited activity to inhibit superoxide anion generation. Both 1 and3, in particular 1, were shown to display significant anti-inflammatory activity by inhibiting the elastase release in FMLP/CB-induced human neutrophils.
Keywords:
Cladiella krempfi; eunicellin-type diterpenoid; anti-inflammatory agent; elastase

1. Introduction

Soft corals have been known to be rich sources of terpenoid metabolites [1]. For the purpose of discovering bioactive agents from marine organisms, we have previously investigated the chemical constituents and reported a series of bioactive natural products from Taiwanese soft corals [2,3,4,5]. In recent studies a series of bioactive eunicellin-based diterpenoids, have been isolated from the soft corals of the genera Cladiella, Klysum and Litophyton sp. [6,7,8,9,10,11,12,13,14]. The soft coral Cladiella krempfi has been found to produce several types of metabolites including eunicellin-type diterpenoids [15,16,17] and pregnane-type steroids [18,19]. Our previous chemical investigation of the Formosan soft coral Cladiella krempfi also resulted in the isolation of a series of new eunicellin-type diterpenoids, krempfielins A–M [20,21,22]. In this paper, we further report the discovery of three new eunicellin-based diterpenoids, krempfielins N–P (13) (Chart 1 and Supplementary Figures S1–S9). The ability of these compounds to inhibit the superoxide anion generation and elastase release in FMLP/CB-induced human neutrophils was also evaluated. The results showed that compound 3 could inhibit superoxide anion generation while 1 and 3, especially 1, effectively inhibited the generation of the elastase release in FMLP/CB-induced human neutrophils.

Marinedrugs 12 01148 g003 200
Chart 1. Structures of metabolites 13.

Click here to enlarge figure

Chart 1. Structures of metabolites 13.
Marinedrugs 12 01148 g003 1024

2. Results and Discussion

The new metabolite krempfielin N (1) showed the molecular ion peak [M + Na]+ at m/z 461.2882 in the HRESIMS and established a molecular formula of C25H42O6, implying five degrees of unsaturation. The IR absorptions bands at νmax 3445 and 1733 cm−1 revealed the presence of hydroxy and ester carbonyl functionalities. The 13C NMR spectrum measured in CDCl3 showed signals of 25 carbons (Table 1) which were assigned by the assistance of the DEPT spectrum to six methyls (including one oxgenate methyl δC 57.0), six sp3 methylenes, one sp2 methylene, eight sp3 methines (including four oxymethines), four quaternary carbons (including one ester carbonyl). The NMR spectroscopic data of 1 (Table 1 and Table 2) showed the presence of one 1,1-disubstituted double bond (δC 112.5 CH2 and 148.0 C; δH 5.03 s, and 4.86 s), one methoxy group (δH 3.34, 3H, s) and one n-butyryloxy group (δC 172.3 C; 37.4 CH2; 18.4 CH2; and 13.7 CH3; δH 2.30 m, 2H; 1.67 m, 2H; and 0.98 t, 3H, J = 7.6 Hz). Therefore, taking account of the two degrees of unsaturation from double bonds, it was suggested that 1 should be a tricyclic compound from the remaining three degrees of unsaturation. The 1H–1H COSY and HMBC correlations (Figure 1) were further used for establishing the molecular skeleton of 1. The COSY experiment assigned three isolated consecutive proton spin systems. Above evidences and the analysis of HMBC spectrum (Figure 1) suggested that 1 is an eunicellin-based diterpenoid. Furthermore, the two hydroxy groups attaching at C-7 and C-12 were confirmed by the HMBC correlations from one methyl (δH 1.12 s, H-16) and one oxymethine (δH 4.12 m, H-6) to the oxygenated quaternary carbon appearing at δ 75.8 (C-7), and one methine (δH 2.91 t, H-10) and one proton of H2-17 (δH 5.03 s) to the oxymethine carbon appearing at δ 71.0 (C-12). Thus, the remaining one n-butyryloxy group had to be positioned at C-3, an oxygen-bearing quaternary carbon resonating at δ 86.5 ppm. On the basis of above analysis, the planar structure of 1 was established. The stereochemistry of 1 was finally confirmed by the very similar NOE correlations of both 1 and krempfielin L [22].

Table 1. 13C NMR data for compounds 13.

Click here to display table

Table 1. 13C NMR data for compounds 13.
1 a2 b3 a
δCδCδC
144.4, CH c45.1, CH43.2, CH c
291.0, CH91.5, CH90.7, CH
386.5, C85.7, C84.5, C
436.0, CH235.7, CH228.7, CH2
526.7, CH228.9, CH235.4, CH2
689.7, CH77.5, CH67.0, CH
775.8, C79.4, C152.5, C
845.2, CH279.0, CH77.2, CH
980.0, CH79.4, CH85.0, CH
1051.4, CH50.1, CH46.6, CH
11148.0, C143.6, C141.4, C
1271.0, CH73.5, CH73.2, CH
1330.7, CH229.2, CH229.0, CH2
1436.6, CH37.2, CH36.9, CH
1523.2, CH323.1, CH322.1, CH3
1623.6, CH318.0, CH3118.1, CH2
17112.5, CH2115.1, CH2119.4, CH2
1828.8, CH28.6, CH26.9, CH
1916.0, CH315.6, CH315.3, CH3
2021.8, CH321.7, CH321.6, CH3
3- n-butyrate172.3, C173.0, C172.5, C
37.4, CH236.7, CH237.4, CH2
18.4, CH218.5, CH218.5, CH2
13.7, CH313.5, CH313.6, CH3
6-OMe57.0, CH3
8-OAc170.7, C
21.4, CH3
12-OAc170.2, C170.1, C
21.6, CH321.7, CH3

a 13C spectra recorded at 100 MHz in CDCl3; b 13C spectra recorded at 125 MHz in CDCl3; c Deduced from DEPT.

Table 2. 1H NMR data for compounds 13.

Click here to display table

Table 2. 1H NMR data for compounds 13.
1 a2 b3 a
δHδHδH
12.25 m2.28 dd (10.0, 7.0)2.26 m
23.70 br s3.67 br s3.84 br s
3
41.86 m, 2.64 m1.85 m, 2.66 m1.68 m, 2.66 m
51.33 m, 1.65 m1.49 m, 1.65 m1.76 m
2.21 m
64.12 m4.66 d (6.5)4.75 dd (10.8, 4.4) d
7
81.82 m5.19 d (10.0)4.20 s
94.53 m4.31 dd (10.0, 6.5)4.43 d (10.8)
102.91 t (6.4) c3.38 dd (7.0, 7.0)2.90 dd (10.8, 8.4)
11
124.39 s5.43 dd (4.0, 3.0)5.49 t (2.8)
131.36 m, 1.86 m1.37 m, 1.93 m1.30 m, 1.98 m
141.86 m1.70 m1.71 m
151.45 s1.45 s1.65 s
161.12 s1.08 s5.23 s, 5.55 s
174.86 s, 5.03 s4.84 s, 5.10 s4.96 d (1.6)
5.27 d (1.6)
181.81 m1.80 m1.96 m
190.82 d (7.6)0.80 d (7.0)0.75 d (6.8)
200.99 d (7.2)0.95 d (7.0)0.95 d (6.8)
3- n-butyrate2.30 m2.60 m, 2.50 m2.12 m
1.67 m1.67 m1.58 m
0.98 t (7.6)1.00 t (7.5)0.92 t (7.6)
6-OMe3.34 s
8-OAc 2.07 s
12-OAc 2.08 s2.05 s

a 1H spectra recorded at 400 MHz in CDCl3; b 1Hspectra recorded at 500 MHz in CDCl3; c J values (Hz) in parentheses.

Marinedrugs 12 01148 g001 200
Figure 1. Selected 1H−1H COSY (▬) and HMBC (→) correlations of 1, 2 and 3.

Click here to enlarge figure

Figure 1. Selected 1H−1H COSY (▬) and HMBC (→) correlations of 1, 2 and 3.
Marinedrugs 12 01148 g001 1024

Krempfielin O (2) was shown by HRESIMS to possess the molecular formula C28H44O9 (m/z 547.2880 [M + Na]+). The NMR spectroscopic data of 2 (Table 1 and Table 2) showed the presence of two acetoxy groups (δH 2.07, s and 2.08, s, each 3H; and δC 170.7, C and 170.2, C; 21.4, CH3 and 21.6, CH3), and an n-butyryloxy group (δH 2.60 m and 2.50 m, each 1H; 1.67 m, 2H and 1.00 t, 3H, J = 7.5 Hz; and δC 173.0, C; 36.7, CH2; 18.5, CH2 and 13.5, CH3). As demonstrated by the HMBC correlation from oxymethine proton H-8 (δ 5.19) to the ester carbonyl carbon appearing at δC 170.7 (Figure 1), one acetoxy group was positioned at C-8. The position of an n-butyryloxy group at C-3 was established by NOE interaction between the methylene protons (δ 1.67) of n-butyryloxy group with H-5 (δ 1.49). The remaining one acetoxy group was thus positioned at C-12. The relative configuration of 2 was further confirmed by NOE correlations (Figure 2).

Marinedrugs 12 01148 g002 200
Figure 2. Key NOESY correlations for 2 and 3.

Click here to enlarge figure

Figure 2. Key NOESY correlations for 2 and 3.
Marinedrugs 12 01148 g002 1024

The related metabolite, krempfielin P (3), had a molecular formula of C26H40O7 as indicated by the HRESIMS (m/z 487.2675, [M + Na]+) and NMR data (Table 1 and Table 2). The 13C NMR spectrum of 3 revealed the appearance of two ester carbonyls (δC 172.5 and 170.1), which were correlated with one methylene (δH 2.12 m, 2H; and δC 37.4) of an n-butyrate and the methyl (δH 2.05 s, 3H; δC 21.7CH3) of an acetate group, respectively. The planar structure of 3 was determined by 1H–1H COSY and HMBC correlations (Figure 1). Comparison of the NMR data of 3 with those of the compound krempfielin A [20] revealed that the only difference is the replacement of one methyl and one hydroxy group at C-7 in krempfielin A by the substitution of one olefinic methylene (δC 118.1, CH2; δH 5.55, s and 5.23, s) in 3. The placement of one n-butyryloxy group and one acetoxy group at C-3 and C-12, respectively was established by comparison of the spectroscopic data with those of krempfielin A. The relative configuration of 3 was mostly determined to be the same as that of krempfielin A by comparison of the chemical shifts of both compounds and was further confirmed by NOE correlations (Figure 2).

Recently, we discovered several eunicellins showed anti-inflammatory activity by significantly inhibiting superoxide anion generation and elastase release in human neutrophiles induced by N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (FMLP/CB) [22,23]. The samein vitro anti-inflammatory effects of the diterpenoids 13 also were tested in this study (Table 3). At a concentration of 10 µM, 1 and 2 could not significantly reduce the generation of superoxide anion, however, 3 inhibited 23.32% ± 5.88% generation of superoxide anion, relative to the control cells stimulated with FMLP/CB only. At the same concentration, all of 13 were found to show anti-inflammatory activity by inhibiting the elastase release. Compound 1 displayed significant inhibition (73.86% ± 14.18%) at this concentration with IC50 of 4.94 ± 1.68 µM in this assay.

Table 3. Effect of pure compounds on elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (FMLP/CB)-induced human neutrophils.

Click here to display table

Table 3. Effect of pure compounds on elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (FMLP/CB)-induced human neutrophils.
CompoundElastase
Inhibition (%)IC50 (µM)
173.86 ± 14.18**4.94 ± 1.68
213.33 ± 3.56*>10
335.54 ± 3.17***>10

Percentage of inhibition (%) was measured at 10 µM; results are presented as mean ± S.E.M. (n = 3 or 4); * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the control value.

3. Experimental Section

3.1. General Experimental Procedures

Melting point was determined using a Fisher-Johns melting point apparatus. Optical rotations were measured on a JASCO P-1020 polarimeter. IR spectra were recorded on a JASCO FT/IR-4100 infrared spectrophotometer. ESIMS were obtained with a Bruker APEX II mass spectrometer. The NMR spectra were recorded either on a Varian UNITY INOVA-500 FT-NMR and a Varian 400MR FT-NMR. Silica gel (Merck, Darmstadt, Germany, 230–400 mesh) was used for column chromatography. Precoated silica gel plates (Merck, Darmstadt, Germany, Kieselgel 60 F-254, 0.2 mm) were used for analytical thin layer chromatography (TLC). High performance liquid chromatography was performed on a Hitachi L-7100 HPLC apparatus with an octadecylsilane (ODS) column (250 × 21.2 mm, 5 µm).

3.2. Animal Material

C. krempfi was collected by hand using scuba off the coast of Penghu islands of Taiwan in June 2008, at a depth of 5–10 m, and stored in a freezer until extraction. A voucher sample (specimen No. 200806CK) was deposited at the Department of Marine Biotechnology and Resources, National Sun Yat-sen University.

3.3. Extraction and Separation

The octocoral (1.1 kg fresh wt) was collected and freeze-dried. The freeze-dried material was minced and extracted exhaustively with EtOH (3 × 10 L). The EtOH extract of the frozen organism was partitioned between CH2Cl2 and H2O. The CH2Cl2-soluble portion (14.4 g) was subjected to column chromatography on silica gel and eluted with EtOAc in n-hexane (0%–100% of EtOAc, stepwise) and then further with MeOH in EtOAc with increasing polarity to yield 41 fractions. Fraction 31, eluted with n-hexane–EtOAc (1:10), was rechromatoraphed over a silica gel open column using n-hexane–acetone (3:1) as the mobile phase to afford eight subfractions (A1–A8). Subfraction A4 was repeatedly separated by reverse phase HPLC (CH3CN–H2O, 0.8:1 to 1:1) to afford compound 1 (3.2 mg). Subfraction A5 separated by reverse phase HPLC (CH3CN–H2O, 1:1 to 1:1.6) to afford compound 2 (1.2 mg). Subfraction A6 by reverse phase HPLC (CH3CN–H2O, 1:1.5) to afford compound 3 (3.9 mg).

3.3.1. Krempfielin N (1)

Colorless oil; Marinedrugs 12 01148 i001 = +27.3 (c 0.91, CHCl3); IR (neat) νmax 3445, 2961, 1733, 1457, 1370, 1180, and 1084 cm−1; 13C and 1H NMR data, see Table 1 and Table 2; ESIMS m/z 461 [M + Na]+; HRESIMS m/z 461.2882 [M + Na]+ (calcd. for C25H42O6Na, 461.2879).

3.3.2. Krempfielin O (2)

Colorless oil; Marinedrugs 12 01148 i001 = −56.7 (c 0.3, CHCl3); IR (neat) νmax 3461, 2960, 1735, 1464, 1372, 1238, 1177, 1076, and 1026 cm−1; 13C and 1H NMR data, see Table 1 and Table 2; ESIMS m/z 547 [M + Na]+; HRESIMS m/z 547.2880 [M + Na]+ (calcd. for C28H44O9Na, 547.2883).

3.3.3. Krempfielin P (3)

Colorless oil; Marinedrugs 12 01148 i001 = +13.1 (c 3.8, CHCl3); IR (neat) νmax 3419, 2959, 1733, 1437, 1371, 1237, 1182, and 1072 cm−1; 13C and 1H NMR data, see Table 1 and Table 2; ESIMS m/z 487 [M + Na]+; HRESIMS m/z 487.2675 [M + Na]+ (calcd for C26H40O7Na, 487.2672).

3.4. In Vitro Anti-Inflammatory Assay—Superoxide Anion Generation and Elastase Release by Human Neutrophils

Human neutrophils were obtained by means of dextran sedimentation and Ficoll centrifugation. Measurements of superoxide anion generation and elastase release were carried out according to previously described procedures [24,25]. LY294002, a phosphatidylinositol-3-kinase inhibitor, was used as a positive control for inhibition of superoxide anion generation and elastase release with IC50 values of 1.88 ± 0.45 and 4.12 ± 0.92 µM, respectively. Briefly, superoxide anion production was assayed by monitoring the superoxide dismutase-inhibitable reduction of ferricytochrome c. Elastase release experiments were performed using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as the elastase substrate.

4. Conclusions

New eunicellin-based diterpenoids were isolated from the soft coral Cladiella krempfi. Compounds 1 and 3, especially 1, could significantly inhibit the release of elastase in FMLP/CB-induced human neutrophils, and 3 inhibited 23% generation of superoxide anion. Thus, compounds 1 and 3 are promising anti-inflammatory agents and may warrant further biomedical investigation.

Acknowledgments

This research was supported by grants from the National Science Council of Taiwan (NSC 102-2113-M-110-001-MY2), Aim for the Top University Program (02C030205) from Ministry of Education of Taiwan and National Sun Yat-sen University–Kaohsiung Medical University Joint Project (NSYSUKMU 02C030117), Taiwan, awarded to Jyh-Horng Sheu.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Blunt, J.W.; Copp, B.R.; Keyzers, R.A.; Munro, M.H.; Prinsep, M.R. Marine natural products. Nat. Prod. Rep. 2013, 30, 237–323, doi:10.1039/c2np20112g.
  2. Lin, W.-Y.; Lu, Y.; Su, J.-H.; Wen, Z.-H.; Dai, C.-F.; Kuo, Y.-H.; Sheu, J.-H. Bioactive cembranoids from the dongsha atoll soft coral Sarcophyton crassocaule. Mar. Drugs 2011, 9, 994–1006, doi:10.3390/md9060994.
  3. Chao, C.-H.; Chou, K.-J.; Huang, C.-Y.; Wen, Z.-H.; Hsu, C.-H.; Wu, Y.-C.; Dai, C.-F.; Sheu, J.-H. Bioactive cembranoids from the soft coral Sinularia crassa. Mar. Drugs 2011, 9, 1955–1968, doi:10.3390/md9101955.
  4. Chao, C.-H.; Chou, K.-J.; Wen, Z.-H.; Wang, G.-H.; Wu, Y.-C.; Dai, C.-F.; Sheu, J.-H. Paraminabeolides A–F, cytotoxic and anti-inflammatory marine withanolides from the soft coral Paraminabea acronocephala. J. Nat. Prod. 2011, 74, 1132–1141, doi:10.1021/np2000705.
  5. Wen, Z.-H.; Chao, C.-H.; Wu, M.-H.; Sheu, J.-H. A neuroprotective sulfone of marine origin and the in vivo anti-inflammatory activity of an analogue. Eur. J. Med. Chem. 2010, 45, 5998–6004, doi:10.1016/j.ejmech.2010.09.067.
  6. Chen, Y.-H.; Tai, C.-Y.; Hwang, T.-L.; Weng, C.-F.; Li, J.-J.; Fang, L.-S.; Wang, W.-H.; Wu, Y.-C.; Sung, P.-J. Cladielloides A and B: New eunicellin-type diterpenoids from an Indonesian octocoral Cladiella sp. Mar. Drugs 2010, 8, 2936–2945, doi:10.3390/md8122936.
  7. Chen, B.-W.; Chang, S.-M.; Huang, C.-Y.; Chao, C.-H.; Su, J.-H.; Wen, Z.-H.; Hsu, C.-H.; Dai, C.-F.; Wu, Y.-C.; Sheu, J.-H. Hirsutalins A–H, eunicellin-based diterpenoids from the soft coral Cladiella hirsuta. J. Nat. Prod. 2010, 73, 1785–1791, doi:10.1021/np100401f.
  8. Hassan, H.M.; Khanfar, M.A.; Elnagar, A.Y.; Mohammed, R.; Shaala, L.A.; Youssef, D.T.A.; Hifnawy, M.S.; El Sayed, K.A. Pachycladins A–E, prostate cancer invasion and migration inhibitory eunicellin-based diterpenoids from the Red Sea soft coral Cladiella pachyclados. J. Nat. Prod. 2010, 73, 848–853, doi:10.1021/np900787p.
  9. Chen, B.-W.; Chao, C.-H.; Su, J.-H.; Wen, Z.-H.; Sung, P.-J.; Sheu, J.-H. Anti-inflammatory eunicellin-based diterpenoids from the cultured soft coral Klyxum simplex. Org. Biomol. Chem. 2010, 8, 2363–2366, doi:10.1039/b926353e.
  10. Chen, Y.-H.; Tai, C.-Y.; Su, Y.-D.; Chang, Y.-C.; Lu, M.-C.; Weng, C.-F.; Su, J.-H.; Hwang, T.-L.; Wu, Y.-C.; Sung, P.-J. Discovery of new eunicellins from an Indonesian octocoral Cladiella sp. Mar. Drugs 2011, 9, 934–943, doi:10.3390/md9060934.
  11. Hsu, F.-J.; Chen, B.-W.; Wen, Z.-H.; Huang, C.-Y.; Dai, C.-F.; Su, J.-H.; Wu, Y.-C.; Sheu, J.-H. Klymollins A–H, bioactive eunicellin-based diterpenoids from the Formosan soft coral Klyxum molle. J. Nat. Prod. 2011, 74, 2467–2471, doi:10.1021/np200589n.
  12. Chen, B.-W.; Chao, C.-H.; Su, J.-H.; Tsai, C.-W.; Wang, W.-H.; Wen, Z.-H.; Huang, C.-Y.; ung, P.-J.; Wu, Y.-C.; Sheu, J.-H. Klysimplexins I–T, eunicellin-based diterpenoids from the cultured soft coral Klyxum simplex. Org. Biomol. Chem. 2011, 9, 834–844, doi:10.1039/c0ob00351d.
  13. Williams, D.E.; Amlani, A.; Dewi, A.S.; Patrict, B.O.; van Ofwegen, L.; Mui, A.L.-F.; Andersen, R.J. Australin E isolated from the soft coral Cladiella sp. collected in Pohnpei activates the inositol 5-phosphatase SHIP1. Aust. J. Chem. 2010, 63, 895–900, doi:10.1071/CH10053.
  14. Iwagawa, T.; Kusatsu, T.; Tsuha, K.; Hamada, T.; Okamura, H.; Furukawa, T.; Akiyama, S.; Doe, M.; Morimoto, Y.; Iwase, F.; et al. Cytotoxic eunicellin-type diterpenes from the soft coral Litophyton viscudium. Heterocycles 2011, 83, 2149–2155, doi:10.3987/COM-11-12251.
  15. Cai, Y.-S.; Yao, L.-G.; Di Pascale, A.; Irace, C.; Mollo, E.; Taglialatela-Scafati, O.; Guo, Y.-W. Polyoxygenated diterpenoids of the eunicellin-type from the Chinese soft coral Cladiella krempfi. Tetrahedron 2013, 69, 2214–2219, doi:10.1016/j.tet.2012.12.051.
  16. Sarma, N.S.; Chavakula, R.; Rao, I.N.; Kadirvelraj, R.; Row, T.N.G.; Saito, I. Crystal and molecular structure of sclerophytin F methyl ether from the soft coral Cladiella krempfi. J. Nat. Prod. 1993, 56, 1977–1980, doi:10.1021/np50101a017.
  17. Lan, W.-J.; Lin, C.-W.; Su, J.-Y.; Zeng, L.-M. Two steroidal glycosides from the soft coral Cladiella krempfi. Chem. J. Chin. Univ. 2003, 24, 2019–2021.
  18. Huang, X.-P.; Deng, Z.-W.; Ofwegen, L.V.; Li, J.; Fu, H.-Z.; Zhu, X.-B.; Lin, W.-H. Two new pregnane glycosides from soft coral Cladiella krempfi. J. Asian Nat. Prod. Res. 2006, 8, 287–291, doi:10.1080/10286020500034782.
  19. Huang, X.-P.; Deng, Z.-W.; Zhu, X.-B.; Ofwegen, L.V.; Proksch, P.; Lin, W.-H. Krempenes A–D: A series of unprecedented pregnane-type steroids from the marine soft coral Cladiella krempfi. Helv. Chim. Acta 2006, 89, 2020–2026, doi:10.1002/hlca.200690192.
  20. Tai, C.-J.; Su, J.-H.; Huang, M.-S.; Wen, Z.-H.; Dai, C.-F.; Sheu, J.-H. Bioactive eunicellin-based diterpenoids from the soft coral Cladiella krempfi. Mar. Drugs 2011, 9, 2036–2045, doi:10.3390/md9102036.
  21. Tai, C.-J.; Su, J.-H.; Huang, C.-Y.; Huang, M.-S.; Wen, Z.-H.; Dai, C.-F.; Sheu, J.-H. Cytotoxic and anti-inflammatory eunicellin-based diterpenoids from the soft coral Cladiella krempfi. Mar. Drugs 2013, 11, 788–799, doi:10.3390/md11030788.
  22. Lee, Y.-N.; Tai, C.-J.; Hwang, T.-L.; Sheu, J.-H. Krempfielins J–M, new eunicellin-based diterpenoids from the soft coral Cladiella krempfi. Mar. Drugs 2013, 11, 2741–2750, doi:10.3390/md11082741.
  23. Lin, M.-C.; Chen, B.-W.; Huang, C.-Y.; Dai, C.-F.; Hwang, T.-L.; Sheu, J.-H. Eunicellin-Based diterpenoids from the Formosan soft coral Klyxum molle with inhibitory activity on superoxide generation and elastase release by neutrophils. J. Nat. Prod. 2013, 76, 1661–1667, doi:10.1021/np400372v.
  24. Yu, H.-P.; Hsieh, P.-W.; Chang, Y.-J.; Chung, P.-J.; Kuo, L.-M.; Hwang, T.-L. 2-(2-Fluorobenzamido) benzoate ethyl ester (EFB-1) inhibits superoxide production by human neutrophils and attenuates hemorrhagic shock-induced organ dysfunction in rats. Free Radic. Biol. Med. 2011, 50, 1737–1748, doi:10.1016/j.freeradbiomed.2011.03.026.
  25. Hwang, T.-L.; Wang, C.-C.; Kuo, Y.-H.; Huang, H.-C.; Wu, Y.-C.; Kuo, L.-M.; Wu, Y.-H. The hederagenin saponin SMG-1 is a natural FMLP receptor inhibitor that suppresses human neutrophil activation. Biochem. Pharmacol. 2010, 80, 1190–1200, doi:10.1016/j.bcp.2010.06.028.
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert