Next Article in Journal
Cyclodextrin Formulation of the Marine Natural Product Pseudopterosin A Uncovers Optimal Pharmacodynamics in Proliferation Studies of Human Umbilical Vein Endothelial Cells
Next Article in Special Issue
Hippuristanol Reduces the Viability of Primary Effusion Lymphoma Cells both in Vitro and in Vivo
Previous Article in Journal
Marine Derived Hamacanthins as Lead for the Development of Novel PDGFRβ Protein Kinase Inhibitors
Previous Article in Special Issue
Towards the Small and the Beautiful: A Small Dibromotyrosine Derivative from Pseudoceratina sp. Sponge Exhibits Potent Apoptotic Effect through Targeting IKK/NFκB Signaling Pathway
Mar. Drugs 2013, 11(9), 3224-3257; doi:10.3390/md11093224
Article

Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1

1,* , 2
, 1,2
, 1,2
, 1,2
, 1,2
, 1,2
, 1,2
, 1,2
, 1,2
 and 3
Received: 23 July 2013; in revised form: 12 August 2013 / Accepted: 20 August 2013 / Published: 26 August 2013
(This article belongs to the Special Issue Marine Compounds and Cancer)
View Full-Text   |   Download PDF [2340 KB, uploaded 26 August 2013]   |   Browse Figures
Abstract: Representatives of Subclass Elasmobranchii are cartilaginous fish whose members include sharks, skates, and rays. Because of their unique phylogenetic position of being the most primitive group of vertebrates to possess all the components necessary for an adaptive immune system, the immune regulatory compounds they possess may represent the earliest evolutionary forms of novel compounds with the potential for innovative therapeutic applications. Conditioned medium, generated from short term culture of cells from the epigonal organ of bonnethead sharks (Sphyrna tiburo), has been shown to have potent reproducible cytotoxic activity against a variety of human tumor cell lines in vitro. Existing data suggest that epigonal conditioned medium (ECM) exerts this cytotoxic activity through induction of apoptosis in target cells. This manuscript describes apoptosis induction in a representative tumor cell line, Jurkat E6-1, in response to treatment with ECM at concentrations of 1 and 2 mg/mL. Data indicate that ECM exposure initiates the mitochondrial pathway of apoptosis through activation of caspase enzymes. Future purification of ECM components may result in the isolation of an immune-regulatory compound with potential therapeutic benefit for treatment of human cancer.
Keywords: epigonal organ; bonnethead shark; Jurkat; apoptosis; tumor cell line epigonal organ; bonnethead shark; Jurkat; apoptosis; tumor cell line
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Walsh, C.J.; Luer, C.A.; Yordy, J.E.; Cantu, T.; Miedema, J.; Leggett, S.R.; Leigh, B.; Adams, P.; Ciesla, M.; Bennett, C.; Bodine, A.B. Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1. Mar. Drugs 2013, 11, 3224-3257.

AMA Style

Walsh CJ, Luer CA, Yordy JE, Cantu T, Miedema J, Leggett SR, Leigh B, Adams P, Ciesla M, Bennett C, Bodine AB. Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1. Marine Drugs. 2013; 11(9):3224-3257.

Chicago/Turabian Style

Walsh, Catherine J.; Luer, Carl A.; Yordy, Jennifer E.; Cantu, Theresa; Miedema, Jodi; Leggett, Stephanie R.; Leigh, Brittany; Adams, Philip; Ciesla, Marissa; Bennett, Courtney; Bodine, Ashby B. 2013. "Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1." Mar. Drugs 11, no. 9: 3224-3257.


Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert