Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Mar. Drugs 2013, 11(12), 5063-5086; doi:10.3390/md11125063
Article

Fumigaclavine C from a Marine-Derived Fungus Aspergillus Fumigatus Induces Apoptosis in MCF-7 Breast Cancer Cells

1
, 1
, 2
, 3
 and 1,2,*
Received: 1 October 2013; in revised form: 30 November 2013 / Accepted: 2 December 2013 / Published: 13 December 2013
(This article belongs to the Special Issue Marine Compounds and Cancer)
View Full-Text   |   Download PDF [1299 KB, uploaded 13 December 2013]
Abstract: Recently, much attention has been given to discovering natural compounds as potent anti-cancer candidates. In the present study, the anti-cancer effects of fumigaclavine C, isolated from a marine-derived fungus, Aspergillus fumigatus, was evaluated in vitro. In order to investigate the impact of fumigaclavine C on inhibition of proliferation and induction of apoptosis in breast cancer, MCF-7 cells were treated with various concentrations of fumigaclavine C, and fumigaclavine C showed significant cytotoxicity towards MCF-7 cells. Anti-proliferation was analyzed via cell mobility and mitogen-activated protein kinase (MAPK) signaling pathway. In addition, fumigaclavine C showed potent inhibition on the protein and gene level expressions of MMP-2, -9 in MCF-7 cells which were manifested in Western blot and reverse transcription polymerase chain reaction (RT-PCR) results. The apoptosis induction abilities of the fumigaclvine C was studied by analyzing the expression of apoptosis related proteins, cell cycle analysis, DNA fragmentation and molecular docking studies. It was found that fumigaclavine C fragmented the MCF-7 cell DNA and arrested the cell cycle by modulating the apoptotic protein expressions. Moreover, fumigaclavine C significantly down-regulated the NF-kappa-B cell survival pathway. Collectively, data suggest that fumigaclavine C has a potential to be developed as a therapeutic candidate for breast cancer.
Keywords: fumigaclavine C; apoptosis; anti-proliferation; mitochondrial pathway; anti-cancer fumigaclavine C; apoptosis; anti-proliferation; mitochondrial pathway; anti-cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Li, Y.-X.; Himaya, S.; Dewapriya, P.; Zhang, C.; Kim, S.-K. Fumigaclavine C from a Marine-Derived Fungus Aspergillus Fumigatus Induces Apoptosis in MCF-7 Breast Cancer Cells. Mar. Drugs 2013, 11, 5063-5086.

AMA Style

Li Y-X, Himaya S, Dewapriya P, Zhang C, Kim S-K. Fumigaclavine C from a Marine-Derived Fungus Aspergillus Fumigatus Induces Apoptosis in MCF-7 Breast Cancer Cells. Marine Drugs. 2013; 11(12):5063-5086.

Chicago/Turabian Style

Li, Yong-Xin; Himaya, S.W.A.; Dewapriya, Pradeep; Zhang, Chen; Kim, Se-Kwon. 2013. "Fumigaclavine C from a Marine-Derived Fungus Aspergillus Fumigatus Induces Apoptosis in MCF-7 Breast Cancer Cells." Mar. Drugs 11, no. 12: 5063-5086.



Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert