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Mar. Drugs 2013, 11(12), 4815-4833; doi:10.3390/md11124815
Article

HPLC-ESI-IT-MS/MS Analysis and Biological Activity of Triterpene Glycosides from the Colombian Marine Sponge Ectyoplasia ferox

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Received: 17 September 2013; in revised form: 28 October 2013 / Accepted: 13 November 2013 / Published: 2 December 2013
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Abstract: The marine sponge Ectyoplasia ferox produces antipredatory and allelopathic triterpenoid glycosides as part of its chemical defense repertoire against predators, competitors, and fouling organisms. These molecules are responsible for the pharmacological potential found in the glycosides present in this species. In order to observe the glycochemical diversity present in E. ferox, a liquid chromatography coupled to a tandem mass spectrometry approach to analyse a complex polar fraction of this marine sponge was performed. This gave valuable information for about twenty-five compounds three of which have been previously reported and another three which were found to be composed of known aglycones. Furthermore, a group of four urabosides, sharing two uncommon substitutions with carboxyl groups at C-4 on the terpenoid core, were identified by a characteristic fragmentation pattern. The oxidized aglycones present in this group of saponins can promote instability, making the purification process difficult. Cytotoxicity, cell cycle modulation, a cell cloning efficiency assay, as well as its hemolytic activity were evaluated. The cytotoxic activity was about IC50 40 µg/mL on Jurkat and CHO-k1 cell lines without exhibiting hemolysis. Discussion on this bioactivity suggests the scanning of other biological models would be worthwhile.
Keywords: Ectyoplasia ferox; marine sponge; triterpenoid glycosides; mass spectrometry Ectyoplasia ferox; marine sponge; triterpenoid glycosides; mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Colorado-Ríos, J.; Muñoz, D.; Montoya, G.; Márquez, D.; Márquez, M.-E.; López, J.; Martínez, A. HPLC-ESI-IT-MS/MS Analysis and Biological Activity of Triterpene Glycosides from the Colombian Marine Sponge Ectyoplasia ferox. Mar. Drugs 2013, 11, 4815-4833.

AMA Style

Colorado-Ríos J, Muñoz D, Montoya G, Márquez D, Márquez M-E, López J, Martínez A. HPLC-ESI-IT-MS/MS Analysis and Biological Activity of Triterpene Glycosides from the Colombian Marine Sponge Ectyoplasia ferox. Marine Drugs. 2013; 11(12):4815-4833.

Chicago/Turabian Style

Colorado-Ríos, Jhonny; Muñoz, Diana; Montoya, Guillermo; Márquez, Diana; Márquez, Maria-Elena; López, Juan; Martínez, Alejandro. 2013. "HPLC-ESI-IT-MS/MS Analysis and Biological Activity of Triterpene Glycosides from the Colombian Marine Sponge Ectyoplasia ferox." Mar. Drugs 11, no. 12: 4815-4833.


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