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Mar. Drugs 2013, 11(12), 4773-4787; doi:10.3390/md11124773

A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China
Department of Pharmacology, Jinan University, Guangzhou 510632, China
Authors to whom correspondence should be addressed.
Received: 11 October 2013 / Revised: 7 November 2013 / Accepted: 12 November 2013 / Published: 2 December 2013
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A new in vitro anti-tumor polypeptide, coded as J2-C3, was isolated from Arca inflata Reeve and purified by diethyl-aminoethanol (DEAE)-sepharose Fast Flow anion exchange and phenyl sepharose CL-4B hydrophobic chromatography. J2-C3 was identified to be a homogeneous compound by native polyacrylamide gel electrophoresis (Native-PAGE). The purity of J2-C3 was over 99% in reversed phase-high performance liquid chromatography (RP-HPLC). The molecular weight was determined as 20,538.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). J2-C3 was rich in Glx (Gln + Glu), Lys, and Asx (Asp + Asn) according to amino acid analysis. Four partial amino acid sequences of this peptide were determined as L/ISMEDVEESR, KNGMHSI/LDVNHDGR, AMKI/LI/LNPKKGI/LVPR and AMGAHKPPKGNEL/IGHR via MALDI-TOF/TOF-MS and de novo sequencing. Secondary structural analysis by CD spectroscopy revealed that J2-C3 had the α-helix (45.2%), β-sheet (2.9%), β-turn (26.0%) and random coil (25.9%). The anti-tumor effect of J2-C3 against human tumor cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC50 values of J2-C3 were 65.57, 93.33 and 122.95 µg/mL against A549, HT-29 and HepG2 cell lines, respectively. Therefore, J2-C3 might be developed as a potential anti-tumor agent.
Keywords: Arca inflata Reeve; polypeptide; structure analysis; anti-tumor activity Arca inflata Reeve; polypeptide; structure analysis; anti-tumor activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Xu, J.; Chen, Z.; Song, L.; Chen, L.; Zhu, J.; Lv, S.; Yu, R. A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata. Mar. Drugs 2013, 11, 4773-4787.

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