Mar. Drugs 2013, 11(12), 4773-4787; doi:10.3390/md11124773

A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

1email, 2email, 2,* email, 1email, 1email, 1email and 1,* email
Received: 11 October 2013; in revised form: 7 November 2013 / Accepted: 12 November 2013 / Published: 2 December 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A new in vitro anti-tumor polypeptide, coded as J2-C3, was isolated from Arca inflata Reeve and purified by diethyl-aminoethanol (DEAE)-sepharose Fast Flow anion exchange and phenyl sepharose CL-4B hydrophobic chromatography. J2-C3 was identified to be a homogeneous compound by native polyacrylamide gel electrophoresis (Native-PAGE). The purity of J2-C3 was over 99% in reversed phase-high performance liquid chromatography (RP-HPLC). The molecular weight was determined as 20,538.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). J2-C3 was rich in Glx (Gln + Glu), Lys, and Asx (Asp + Asn) according to amino acid analysis. Four partial amino acid sequences of this peptide were determined as L/ISMEDVEESR, KNGMHSI/LDVNHDGR, AMKI/LI/LNPKKGI/LVPR and AMGAHKPPKGNEL/IGHR via MALDI-TOF/TOF-MS and de novo sequencing. Secondary structural analysis by CD spectroscopy revealed that J2-C3 had the α-helix (45.2%), β-sheet (2.9%), β-turn (26.0%) and random coil (25.9%). The anti-tumor effect of J2-C3 against human tumor cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC50 values of J2-C3 were 65.57, 93.33 and 122.95 µg/mL against A549, HT-29 and HepG2 cell lines, respectively. Therefore, J2-C3 might be developed as a potential anti-tumor agent.
Keywords: Arca inflata Reeve; polypeptide; structure analysis; anti-tumor activity
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MDPI and ACS Style

Xu, J.; Chen, Z.; Song, L.; Chen, L.; Zhu, J.; Lv, S.; Yu, R. A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata. Mar. Drugs 2013, 11, 4773-4787.

AMA Style

Xu J, Chen Z, Song L, Chen L, Zhu J, Lv S, Yu R. A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata. Marine Drugs. 2013; 11(12):4773-4787.

Chicago/Turabian Style

Xu, Jian; Chen, Zhiyan; Song, Liyan; Chen, Lili; Zhu, Jianhua; Lv, Shuangshuang; Yu, Rongmin. 2013. "A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata." Mar. Drugs 11, no. 12: 4773-4787.

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