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Sensors 2008, 8(9), 5492-5500; doi:10.3390/s8095492

An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film

Department of Chemistry, Faculty of Arts and Sciences, Gazi University, 06500 Ankara, Turkey
Received: 17 July 2008 / Revised: 14 August 2008 / Accepted: 26 August 2008 / Published: 4 September 2008
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Abstract

A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 – 8.5×10-5 M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 oC, respectively. The stability and reproducibility of the enzyme electrode have been also studied. View Full-Text
Keywords: Uric acid; Uricase; Biosensor; Polypyrrole; Polyaniline Uric acid; Uricase; Biosensor; Polypyrrole; Polyaniline
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MDPI and ACS Style

Arslan, F. An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film. Sensors 2008, 8, 5492-5500.

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