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Diversity 2014, 6(1), 33-42; doi:10.3390/d6010033

Novel Microsatellite Loci Variation and Population Genetics within Leafy Seadragons, Phycodurus eques

Seattle Aquarium, 1483 Alaskan Way, Pier 59, Seattle, WA 98101, USA
Department of Clinical Investigation, Madigan Army Medical Center, 9040 Fitzsimmons Dr., Tacoma, WA 98431, USA
Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 2 October 2013 / Revised: 20 December 2013 / Accepted: 27 December 2013 / Published: 3 January 2014
(This article belongs to the Special Issue Genetic Diversity and Molecular Evolution)
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Novel leafy seadragon (Phycodurus eques) microsatellite loci were developed via standard cloning techniques and tested for use in population genetics studies. Six out of a total of twelve microsatellites tested were usable for population analysis. Seadragon samples from Western Australia (N = 6), Southern Australia (N = 11), and a captive group (N = 11) were analyzed. Here, we present leafy seadragon microsatellite primer sequences for all 12 loci and population genetics statistics for the six loci that amplified consistently and displayed adequate variability to estimate population parameters, such as diversity, population differences, and relatedness. Observed heterozygosities ranged from 0.225 to 0.926 and expected heterozygosities ranged from 0.278 to 0.650. Pairwise differences among populations (FST estimates) from samples collected off the southern coast of Western and South Australia, and captive animals ranged from a low of 0.188 between Southern Australia and captive animals, to a high of 0.212 between Western Australia and captive animals. Statistical assignment analyses suggested between one and three populations. Percent first order relatives among individuals was high and ranged from 40 within Western Australia to 55 within captive animals. These loci were tested on other species including weedy seadragons (Phyllopteryx taeniolatus), as well as assorted seahorses (Hippocampus reidi, H. erectus) and pipefish (Doryrhamphus dactyliophorus, D. pessuliferus, Corythoichthys intestinalis, Syngnathus leptorhynchus) with no success.
Keywords: seadragons; microsatellites; diversity; seahorses seadragons; microsatellites; diversity; seahorses
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Larson, S.; Ramsey, C.; Tinnemore, D.; Amemiya, C. Novel Microsatellite Loci Variation and Population Genetics within Leafy Seadragons, Phycodurus eques. Diversity 2014, 6, 33-42.

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