Int. J. Mol. Sci. 2002, 3(10), 1058-1072; doi:10.3390/i3101058

Functionally-Impaired HIV-1 Nef Alleles from a Mother-Child Transmission Pair

1,* email, 1, 5, Jr. 2, 3, 4, 1 and 1
Received: 7 June 2002; Accepted: 31 October 2002 / Published: 31 October 2002
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Unusual HIV-1 nef alleles were isolated from a woman and her vertically infected child. Both patients eventually progressed to develop AIDS. The child died at age 6.5 years, while the mother is currently alive, 13 years since her diagnosis with HIV-1. Predicted amino acid sequences of both mother and child Nefs diverged from the HIV-1 clade B consensus. In particular, they exhibited two separate 5-amino acid deletions bracketing a Cterminal dileucine regulatory motif and Trp-Gly mutations at the site for cleavage by the HIV-1 protease. The child’s Nef showed a modest ability to enhance HIV-1 infectivity in MAGI cells, whereas the mother’s Nef did not alter HIV-1 infectivity in the assay. Both Nefs were partially functional for CD4 down-regulation. The child’s Nef was fully functional for MHC-1 down-regulation, while the maternal Nef was non-functional. To our knowledge this study is the first to describe a functional divergence between Nef alleles in a case of mother-to-child HIV-1 transmission.
Keywords: HIV-1; Nef; in vitro; CD4; MHC; infectivity
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MDPI and ACS Style

Roth, W.W.; Khan, M.; Geleziunas, R.; Stringer, H.G., Jr.; Zuberi, J.A.; Greene, W.C.; Powell, M.; Bond, V.C. Functionally-Impaired HIV-1 Nef Alleles from a Mother-Child Transmission Pair. Int. J. Mol. Sci. 2002, 3, 1058-1072.

AMA Style

Roth WW, Khan M, Geleziunas R, Stringer HG, Jr, Zuberi JA, Greene WC, Powell M, Bond VC. Functionally-Impaired HIV-1 Nef Alleles from a Mother-Child Transmission Pair. International Journal of Molecular Sciences. 2002; 3(10):1058-1072.

Chicago/Turabian Style

Roth, William W.; Khan, Mafhuz; Geleziunas, Romas; Stringer, Harold G., Jr.; Zuberi, Jalal A.; Greene, Warner C.; Powell, Michael; Bond, Vincent C. 2002. "Functionally-Impaired HIV-1 Nef Alleles from a Mother-Child Transmission Pair." Int. J. Mol. Sci. 3, no. 10: 1058-1072.

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