Serum-Resistant Ternary DNA Polyplexes for Suicide Gene Therapy of Uterine Leiomyoma
Abstract
:1. Introduction
2. Results and Discussion
2.1. Design of the Carriers
2.2. Assessment of pDNA Binding and Relaxation of Polyplexes by Dextran Sulfate
2.3. Disulfide Bonds Formation during Template Polymerization
2.4. Measurement of Size and Z-Potential of Ternary Polyplexes
2.5. Assessment of Cytotoxicity
2.6. Transfection Studies
2.7. Measurement of DNA Uptake
2.8. In Vitro Suicide Gene Therapy of Uterine Leiomyoma
2.9. In Situ Gene Delivery of Reporter and HSV-TK Genes in UL Organotypc Model
3. Materials and Methods
3.1. Cell Lines and Organotypic Model of Uterine Leiomyoma
3.2. Peptide Carrier Preparation and Characterization
3.3. Plasmids
3.4. Preparation of Carrier/DNA Complexes
3.5. DNA Binding and Relaxation of Polyplexes by Dextran-Sulfate
3.6. Ellman’s Assay
3.7. Size and Z-Potential Measurements, Transmission Electronic Microscopy
3.8. Gene Transfer and Cytotoxicity Assays
3.9. Cellular Uptake of Peptide/DNA Complexes
3.10. In Vitro Suicide Gene Therapy of Uterine Leiomyoma
3.11. In Situ Gene Transfer to Myomatous Nodes
3.12. RNA Extraction and RT-qPCR
3.13. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Composition | |
---|---|---|
Monomers | R6 | CHRRRRRRHC |
E6 | CHEEEEEEHC | |
Ligand monomer | cRGD | C(Npys)RGDy |__________| |
Polycondensed carriers | R6p | (CHRRRRRRHC)n |
R6p-cRGD | cRGD-(CHRRRRRRHC)n-cRGD | |
E6p | (CHEEEEEEHC)n | |
E6p-cRGD | cRGD-(CHEEEEEEHC)n-cRGD |
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Egorova, A.; Shtykalova, S.; Maretina, M.; Freund, S.; Selutin, A.; Shved, N.; Selkov, S.; Kiselev, A. Serum-Resistant Ternary DNA Polyplexes for Suicide Gene Therapy of Uterine Leiomyoma. Int. J. Mol. Sci. 2024, 25, 34. https://doi.org/10.3390/ijms25010034
Egorova A, Shtykalova S, Maretina M, Freund S, Selutin A, Shved N, Selkov S, Kiselev A. Serum-Resistant Ternary DNA Polyplexes for Suicide Gene Therapy of Uterine Leiomyoma. International Journal of Molecular Sciences. 2024; 25(1):34. https://doi.org/10.3390/ijms25010034
Chicago/Turabian StyleEgorova, Anna, Sofia Shtykalova, Marianna Maretina, Svetlana Freund, Alexander Selutin, Natalia Shved, Sergei Selkov, and Anton Kiselev. 2024. "Serum-Resistant Ternary DNA Polyplexes for Suicide Gene Therapy of Uterine Leiomyoma" International Journal of Molecular Sciences 25, no. 1: 34. https://doi.org/10.3390/ijms25010034