Combined Metabolome and Transcriptome Analysis Highlights the Host’s Influence on Cistanche deserticola Metabolite Accumulation
Abstract
:1. Introduction
2. Results
2.1. Metabolic Profiling of C. deserticola Fleshy Stems and H. ammodendron Roots
2.2. Differently Accumulated Metabolites in C. deserticola Fleshy Stems and H. ammodendron Roots
2.3. Phenylethanoid Glycosides and Their Precursors in C. deserticola Fleshy Stems and H. ammodendron Roots
2.4. Transcriptomic Changes Caused by Parasitism of C. deserticola in H. ammodendron Roots
2.5. Genes Specifically Upregulated in Haustoria
2.6. Integrated Analysis of Metabolites and Transcripts Linked to Phenylethanoid Glycoside Biosynthesis in H. ammodendron Roots
2.7. Identification and Phylogenetic Analysis of UGTs in C. deserticola and H. ammodendron
2.8. UGT Candidates Involved in Phenylethanoid Glycoside Biosynthesis
2.9. Quantitative RT-PCR Validation for C. deserticola and H. ammodendron Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Analysis of Metabolites in C. deserticola Fleshy Stems and H. ammodendron Roots
4.3. Screening of Differentially Accumulated Metabolites
4.4. RNA Extraction and Illumina Sequencing
4.5. RNA-Seq Analysis of C. deserticola and H. ammodendron
4.6. Correlation Analysis of Inigenes and Metabolites Linked to PhG Biosynthesis
4.7. Identification and Phylogenetic Analysis of UGTs
4.8. Quantitative RT-PCR Validation of Differential Gene Expression
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample ID | Species | Harvesting Time | Tissue | |
---|---|---|---|---|
CF | CF1 | C. deserticola | November 2019 | Fleshy stem, 5 cm from haustorium |
CF2 | ||||
CF3 | ||||
HcF | HcF1 | H. ammodendron | Root intruded by C. deserticola, 10 cm from haustorium | |
HcF2 | ||||
HcF3 | ||||
HF | HF1 | Root of healthy plant | ||
HF2 | ||||
HF3 | ||||
CS | CS1 | C. deserticola | August 2020 | Fleshy stem, 5 cm from haustorium |
CS2 | ||||
CS3 | ||||
HcS | HcS1 | H. ammodendron | Root intruded by C. deserticola, 10 cm from haustorium | |
HcS2 | ||||
HcS3 | ||||
HS | HS1 | Root of healthy plant | ||
HS2 | ||||
HS3 | ||||
XS | XS1 | C. deserticola and H. ammodendron | Haustorium, the connecting place of C. deserticola and H. ammodendron, with a thickness of 0.5 cm | |
XS2 | ||||
XS3 |
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Feng, R.; Wei, H.; Xu, R.; Liu, S.; Wei, J.; Guo, K.; Qiao, H.; Xu, C. Combined Metabolome and Transcriptome Analysis Highlights the Host’s Influence on Cistanche deserticola Metabolite Accumulation. Int. J. Mol. Sci. 2023, 24, 7968. https://doi.org/10.3390/ijms24097968
Feng R, Wei H, Xu R, Liu S, Wei J, Guo K, Qiao H, Xu C. Combined Metabolome and Transcriptome Analysis Highlights the Host’s Influence on Cistanche deserticola Metabolite Accumulation. International Journal of Molecular Sciences. 2023; 24(9):7968. https://doi.org/10.3390/ijms24097968
Chicago/Turabian StyleFeng, Ru, Hongshuang Wei, Rong Xu, Sai Liu, Jianhe Wei, Kun Guo, Haili Qiao, and Changqing Xu. 2023. "Combined Metabolome and Transcriptome Analysis Highlights the Host’s Influence on Cistanche deserticola Metabolite Accumulation" International Journal of Molecular Sciences 24, no. 9: 7968. https://doi.org/10.3390/ijms24097968