Endogenous DNase Activity in an Animal Model of Acute Liver Failure
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Real-Time PCR
4.3. DNase Activity
4.4. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Sisirak, V.; Sally, B.; D’Agati, V.; Martinez-Ortiz, W.; Ozcakar, Z.B.; David, J.; Rashidfarrokhi, A.; Yeste, A.; Panea, C.; Chida, A.S.; et al. Digestion of Chromatin in Apoptotic Cell Microparticles Prevents Autoimmunity. Cell 2016, 166, 88–101. [Google Scholar] [CrossRef]
- Kishi, K.; Yasuda, T.; Takeshita, H. DNase I: Structure, function, and use in medicine and forensic science. Leg. Med. 2001, 3, 69–83. [Google Scholar] [CrossRef]
- Vokalova, L.; Laukova, L.; Conka, J.; Meliskova, V.; Borbelyova, V.; Babickova, J.; Tothova, L.; Hodosy, J.; Vlkova, B.; Celec, P. Deoxyribonuclease partially ameliorates thioacetamide-induced hepatorenal injury. Am. J. Physiology. Gastrointest. Liver Physiol. 2017, 312, G457–G463. [Google Scholar] [CrossRef]
- Lehmann-Werman, R.; Magenheim, J.; Moss, J.; Neiman, D.; Abraham, O.; Piyanzin, S.; Zemmour, H.; Fox, I.; Dor, T.; Grompe, M.; et al. Monitoring liver damage using hepatocyte-specific methylation markers in cell-free circulating DNA. JCI Insight 2018, 3, e120687. [Google Scholar] [CrossRef]
- Fan, H.C.; Blumenfeld, Y.J.; Chitkara, U.; Hudgins, L.; Quake, S.R. Analysis of the size distributions of fetal and maternal cell-free DNA by paired-end sequencing. Clin. Chem. 2010, 56, 1279–1286. [Google Scholar] [CrossRef] [PubMed]
- Wen, Z.; Lei, Z.; Yao, L.; Jiang, P.; Gu, T.; Ren, F.; Liu, Y.; Gou, C.; Li, X.; Wen, T. Circulating histones are major mediators of systemic inflammation and cellular injury in patients with acute liver failure. Cell Death Dis. 2016, 7, e2391. [Google Scholar] [CrossRef]
- Zhang, X.; Wu, X.; Hu, Q.; Wu, J.; Wang, G.; Hong, Z.; Ren, J.; Lab for Trauma and Surgical Infections. Mitochondrial DNA in liver inflammation and oxidative stress. Life Sci. 2019, 236, 116464. [Google Scholar] [CrossRef]
- Lazaro-Ibanez, E.; Lasser, C.; Shelke, G.V.; Crescitelli, R.; Jang, S.C.; Cvjetkovic, A.; Garcia-Rodriguez, A.; Lotvall, J. DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology. J. Extracell. Vesicles 2019, 8, 1656993. [Google Scholar] [CrossRef]
- Konecna, B.; Tothova, L.; Repiska, G. Exosomes-Associated DNA-New Marker in Pregnancy Complications? Int. J. Mol. Sci. 2019, 20, 2890. [Google Scholar] [CrossRef] [PubMed]
- Meddeb, R.; Dache, Z.A.A.; Thezenas, S.; Otandault, A.; Tanos, R.; Pastor, B.; Sanchez, C.; Azzi, J.; Tousch, G.; Azan, S.; et al. Quantifying circulating cell-free DNA in humans. Sci. Rep. 2019, 9, 5220. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Janovicova, L.; Konecna, B.; Vokalova, L.; Laukova, L.; Vlkova, B.; Celec, P. Sex, Age, and Bodyweight as Determinants of Extracellular DNA in the Plasma of Mice: A Cross-Sectional Study. Int. J. Mol. Sci. 2019, 20, 4163. [Google Scholar] [CrossRef] [PubMed]
- Jimenez-Alcazar, M.; Rangaswamy, C.; Panda, R.; Bitterling, J.; Simsek, Y.J.; Long, A.T.; Bilyy, R.; Krenn, V.; Renne, C.; Renne, T.; et al. Host DNases prevent vascular occlusion by neutrophil extracellular traps. Science 2017, 358, 1202–1206. [Google Scholar] [CrossRef] [PubMed]
- Serpas, L.; Chan, R.W.Y.; Jiang, P.; Ni, M.; Sun, K.; Rashidfarrokhi, A.; Soni, C.; Sisirak, V.; Lee, W.S.; Cheng, S.H.; et al. Dnase1l3 deletion causes aberrations in length and end-motif frequencies in plasma DNA. Proc. Natl. Acad. Sci. USA 2019, 116, 641–649. [Google Scholar] [CrossRef]
- Marques, P.E.; Oliveira, A.G.; Pereira, R.V.; David, B.A.; Gomides, L.F.; Saraiva, A.M.; Pires, D.A.; Novaes, J.T.; Patricio, D.O.; Cisalpino, D.; et al. Hepatic DNA deposition drives drug-induced liver injury and inflammation in mice. Hepatology 2015, 61, 348–360. [Google Scholar] [CrossRef]
- Staynov, D.Z. DNase I digestion reveals alternating asymmetrical protection of the nucleosome by the higher order chromatin structure. Nucleic Acids Res. 2000, 28, 3092–3099. [Google Scholar] [CrossRef] [PubMed]
- Zaborowski, M.P.; Balaj, L.; Breakefield, X.O.; Lai, C.P. Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study. Bioscience 2015, 65, 783–797. [Google Scholar] [CrossRef]
- Riley, J.S.; Tait, S.W. Mitochondrial DNA in inflammation and immunity. EMBO Rep. 2020, 21, e49799. [Google Scholar] [CrossRef]
- Nakahira, K.; Hisata, S.; Choi, A.M. The Roles of Mitochondrial Damage-Associated Molecular Patterns in Diseases. Antioxid Redox Signal 2015, 23, 1329–1350. [Google Scholar] [CrossRef] [PubMed]
- Kustanovich, A.; Schwartz, R.; Peretz, T.; Grinshpun, A. Life and death of circulating cell-free DNA. Cancer Biol. Ther. 2019, 20, 1057–1067. [Google Scholar] [CrossRef]
- Babickova, J.; Conka, J.; Janovicova, L.; Boris, M.; Konecna, B.; Gardlik, R. Extracellular DNA as a Prognostic and Therapeutic Target in Mouse Colitis under DNase I Treatment. Folia Biol. 2018, 64, 10–15. [Google Scholar]
- Mai, S.H.; Khan, M.; Dwivedi, D.J.; Ross, C.A.; Zhou, J.; Gould, T.J.; Gross, P.L.; Weitz, J.I.; Fox-Robichaud, A.E.; Liaw, P.C.; et al. Delayed but not Early Treatment with DNase Reduces Organ Damage and Improves Outcome in a Murine Model of Sepsis. Shock 2015, 44, 166–172. [Google Scholar] [CrossRef] [PubMed]
- Andriamanampisoa, C.L.; Bancaud, A.; Boutonnet-Rodat, A.; Didelot, A.; Fabre, J.; Fina, F.; Garlan, F.; Garrigou, S.; Gaudy, C.; Ginot, F.; et al. BIABooster: Online DNA Concentration and Size Profiling with a Limit of Detection of 10 fg/muL and Application to High-Sensitivity Characterization of Circulating Cell-Free DNA. Anal. Chem. 2018, 90, 3766–3774. [Google Scholar] [CrossRef] [PubMed]
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Janovičová, Ľ.; Kmeťová, K.; Pribulová, N.; Janko, J.; Gromová, B.; Gardlík, R.; Celec, P. Endogenous DNase Activity in an Animal Model of Acute Liver Failure. Int. J. Mol. Sci. 2023, 24, 2984. https://doi.org/10.3390/ijms24032984
Janovičová Ľ, Kmeťová K, Pribulová N, Janko J, Gromová B, Gardlík R, Celec P. Endogenous DNase Activity in an Animal Model of Acute Liver Failure. International Journal of Molecular Sciences. 2023; 24(3):2984. https://doi.org/10.3390/ijms24032984
Chicago/Turabian StyleJanovičová, Ľubica, Katarína Kmeťová, Nikola Pribulová, Jakub Janko, Barbora Gromová, Roman Gardlík, and Peter Celec. 2023. "Endogenous DNase Activity in an Animal Model of Acute Liver Failure" International Journal of Molecular Sciences 24, no. 3: 2984. https://doi.org/10.3390/ijms24032984