Characterization of the IAA-Producing and -Degrading Pseudomonas Strains Regulating Growth of the Common Duckweed (Lemna minor L.)
Abstract
:1. Introduction
2. Results
2.1. Analysis of Bacterial Ability to Utilize IAA as A Sole Carbon Source and Evolutionary Relations between IAA-Producing and -Utilizing Bacterial Strains
2.2. Bacterial Viability after H2O2 and IAA Treatment
2.3. Biofilm Formation and Chemotaxis
2.4. HPLC-DAD Determination of IAA
2.5. Co-Cultivation of Bacteria and Duckweeds
3. Discussion
3.1. Bacterial Production of IAA
3.2. Bacterial Degradation of IAA
3.3. IAA and Bacterial Viability under Oxidative Stress, Biofilm Formation and Chemotaxis
3.4. Effects of Bacterial Strains and Exogenous IAA on Doubling Time of Duckweeds
4. Material and Methods
4.1. Plant Material and Growth Conditions
4.2. Initial IAA Screening of Bacterial Isolates Associated with the Rhizosphere of L. minor: The Microwell Method
4.3. Bacterial Growth in Minimal M9 Medium with IAA as the Sole Carbon Source
4.4. Bacterial Degradation of IAA
4.5. Bacterial Viability after H2O2 and IAA Treatment
4.6. Biofilm Formation
4.7. Soft Agar Swim Assay
4.8. Extract Preparation and HPLC-DAD Determination of IAA
4.9. Co-Cultivation of Selected Bacterial Strains and Duckweeds
4.10. Phylogenetic Analysis
4.11. Statistical Analysis and Graphical Presentation of the Results
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Correction Statement
References
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Annotation | Strain | Species | Accession Number |
---|---|---|---|
15 | D1-104/3 | Pseudomonas oryzihabitans | MF526913 |
23 | A3-104/5 | Pseudomonas putida | MF526920 |
38 | C31-106/3 | Pseudomonas gessardii | MF526935 |
47 | C44-104/1 | Pseudomonas yamanorum | MF526941 |
Strain | Generation Time (h) | Growth Rate (h−1) | Relative Yield (OD600 Units Per 1 mmol of IAA) |
---|---|---|---|
P. oryzihabitans D1-104/3 | 7.64 ± 0.08 | 0.09 ± 0.01 | 0.08 ± 0.02 |
P. putida A3-104/5 | 5.60 ± 0.05 | 0.12 ± 0.02 | 0.05 ± 0.01 |
P. gessardii C31-106/3 | N/A | N/A | N/A |
P. yamanorum C44-104/1 | 6.58 ± 0.05 | 0.10 ± 0.01 | 0.11 ± 0.05 |
Strain | IAA Content (mg g−1 DW) | IAA Content (mg OD600−1) |
---|---|---|
P. oryzihabitans D1-104/3 | 7.41 ± 0.05 | 2.774 ± 0.005 |
P. putida A3-104/5 | 5.47 ± 0.07 | 1.936 ± 0.005 |
P. gessardii C31-106/3 | 1.12 ± 0.06 | 0.386 ± 0.009 |
P. yamanorum C44-104/1 | 1.16 ± 0.05 | 0.626 ± 0.005 |
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Popržen, T.; Nikolić, I.; Krstić-Milošević, D.; Uzelac, B.; Trifunović-Momčilov, M.; Marković, M.; Radulović, O. Characterization of the IAA-Producing and -Degrading Pseudomonas Strains Regulating Growth of the Common Duckweed (Lemna minor L.). Int. J. Mol. Sci. 2023, 24, 17207. https://doi.org/10.3390/ijms242417207
Popržen T, Nikolić I, Krstić-Milošević D, Uzelac B, Trifunović-Momčilov M, Marković M, Radulović O. Characterization of the IAA-Producing and -Degrading Pseudomonas Strains Regulating Growth of the Common Duckweed (Lemna minor L.). International Journal of Molecular Sciences. 2023; 24(24):17207. https://doi.org/10.3390/ijms242417207
Chicago/Turabian StylePopržen, Tatjana, Ivan Nikolić, Dijana Krstić-Milošević, Branka Uzelac, Milana Trifunović-Momčilov, Marija Marković, and Olga Radulović. 2023. "Characterization of the IAA-Producing and -Degrading Pseudomonas Strains Regulating Growth of the Common Duckweed (Lemna minor L.)" International Journal of Molecular Sciences 24, no. 24: 17207. https://doi.org/10.3390/ijms242417207