Targeting Heat Shock Protein 27 and Fatty Acid Oxidation Augments Cisplatin Treatment in Cisplatin-Resistant Ovarian Cancer Cell Lines
Abstract
:1. Introduction
2. Results
2.1. HSP27 Inhibits Cisplatin-Induced ROS and Ferroptosis in Cisplatin-Resistant Ovarian Cancer Cells
2.2. ROS Depletion Attenuates HSP27 Induction and Cytotoxic Effects of Cisplatin
2.3. HSP27-Knockdown Ovarian Cancer Cells Exhibit Decreased Levels of GSH and G6PD
2.4. HSP27-Knockdown Ovarian Cancer Cells Exhibit Increased Levels of Lipid Peroxidation, and NAC Attenuates Cisplatin-Induced FAO Marker Upregulation
2.5. Dual Inhibition of HSP27 and FAO Augments the Antitumor Effect of Cisplatin In Vivo
2.6. Differential Expression of HSP27 and CPT1A in A2780CIS Xenograft Tumors
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Viability Assays
4.3. Lentiviral shRNA Knockdown
4.4. Immunoblotting
4.5. Xenograft Experiment
4.6. Immunohistochemistry
4.7. Flow Cytometry
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Heiserman, J.P.; Minhas, Z.; Nikpayam, E.; Cheon, D.-J. Targeting Heat Shock Protein 27 and Fatty Acid Oxidation Augments Cisplatin Treatment in Cisplatin-Resistant Ovarian Cancer Cell Lines. Int. J. Mol. Sci. 2023, 24, 12638. https://doi.org/10.3390/ijms241612638
Heiserman JP, Minhas Z, Nikpayam E, Cheon D-J. Targeting Heat Shock Protein 27 and Fatty Acid Oxidation Augments Cisplatin Treatment in Cisplatin-Resistant Ovarian Cancer Cell Lines. International Journal of Molecular Sciences. 2023; 24(16):12638. https://doi.org/10.3390/ijms241612638
Chicago/Turabian StyleHeiserman, James Patrick, Zenab Minhas, Elahe Nikpayam, and Dong-Joo Cheon. 2023. "Targeting Heat Shock Protein 27 and Fatty Acid Oxidation Augments Cisplatin Treatment in Cisplatin-Resistant Ovarian Cancer Cell Lines" International Journal of Molecular Sciences 24, no. 16: 12638. https://doi.org/10.3390/ijms241612638