Impact of Fluid Dynamics on the Viability and Differentiation Capacity of 3D-Cultured Jaw Periosteal Cells
Abstract
:1. Introduction
2. Results
2.1. Cell Proliferation within JPC-Seeded Scaffolds Cultured in the Perfusion System
2.2. Visualization and Quantitative Analysis of Cell Distribution within β-TCP Scaffolds Cultured under Static and Perfusion Conditions by Crystal Violet Staining
2.3. Visualization of Cell Morphology on JPC-Seeded β-TCP Scaffolds and Quantification of Scaffold Porosity by Scanning Electron Microscopy
2.4. Visualization of Cell Density and Distribution within Scaffolds by Fluorescent Staining and Microscopy
2.5. Gene Expression Analyses of Osteogenic Markers in 3D-Cultured JPCs Growing under Perfusion Conditions
3. Discussion
4. Materials and Methods
4.1. Isolation and Expansion of Jaw Periosteal Cells (JPCs)
4.2. Cell Seeding of β-TCP Scaffolds
4.3. Cultivation and Configuration of the Perfusion Bioreactor
4.4. Cell Metabolic Activity Assay
4.5. Crystal Violet Staining and Quantification
4.6. Scanning Electron Microscope (SEM) Analysis of JPC-Seeded Scaffolds
4.7. Embedding and Microtome Sectioning of Embedded JPC-Seeded Scaffolds
4.8. Fluorescent Cell Labeling with Sytox Orange
4.9. Gene Expression Analyses by Quantitative PCR
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cen, W.; Wang, S.; Umrath, F.; Reinert, S.; Alexander, D. Impact of Fluid Dynamics on the Viability and Differentiation Capacity of 3D-Cultured Jaw Periosteal Cells. Int. J. Mol. Sci. 2022, 23, 4682. https://doi.org/10.3390/ijms23094682
Cen W, Wang S, Umrath F, Reinert S, Alexander D. Impact of Fluid Dynamics on the Viability and Differentiation Capacity of 3D-Cultured Jaw Periosteal Cells. International Journal of Molecular Sciences. 2022; 23(9):4682. https://doi.org/10.3390/ijms23094682
Chicago/Turabian StyleCen, Wanjing, Suya Wang, Felix Umrath, Siegmar Reinert, and Dorothea Alexander. 2022. "Impact of Fluid Dynamics on the Viability and Differentiation Capacity of 3D-Cultured Jaw Periosteal Cells" International Journal of Molecular Sciences 23, no. 9: 4682. https://doi.org/10.3390/ijms23094682