The Transition of Photoreceptor Guanylate Cyclase Type 1 to the Active State
, Alexander Scholten 1, Ilia A. Solov’yov 2,3 and Karl-Wilhelm Koch 1,3,*Round 1
Reviewer 1 Report
Comments for author File: 
Comments.docx
Reviewer 2 Report
The article describes an attempt to uncover the molecular mechanism of GC activation. Authors checked the hypothesis whether the photoreceptor GC-E undergoes an α-helix rotation when reaching the active state. The approach by integration of alanine residues close to the transmembrane region is adequate, and the results with no doubt shows that α-helix rotation in GC-E is not involved in activation. Instead, authors suggest that the swinging movement of the dimerization domain in the V902L mutant might represent the critical conformational switch in GC going from the low-to-high activity state. Overall, results show a principal difference in the activation modus between hormone receptor GCs and sensory GCs. The article is well written, the objectives, methods, and conclusions are clear. The paper can be published in present form .
Reviewer 3 Report
The manuscript entitled “The Transition of Photoreceptor Guanylate Cyclase Type 1 to 2 the Active State” is a fairly well written article, where Shahu et al, covered all the basic experiments to study active stated of GCT1. Therefore, I recommend this manuscript for publication.
