How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels
Abstract
:1. Introduction
2. MscS and MscS-like Channels
2.1. Architecture of MscS
2.2. Structure of YnaI and Further Larger MscS-like Homologs
Channel | Organism | # TM Helices/SU | # Amino Acids/SU | Conductance (nS) | PDB | EMDB | Reconstitution | Putative State | Observed Lipids | Mutant? | Struct. Ref. |
---|---|---|---|---|---|---|---|---|---|---|---|
MscS | Helicobacter pylori | 3 | 274 | n.r. | 4HW9 | - | DDM | Closed | - | - | [33] |
MscS | Thermoanaerobacter tencongensis | 3 | 282 | 0.05–0.13 [29,52] | 3T9N | - | Triton-X100 | Closed | - | - | [29] |
MscS | Escherichia coli | 3 | 286 | 1.25 [28,39] | 2OAU | - | Fos-choline 14 | Closed | - | - | [26,27] |
2VV5 | - | Fos-choline 14 | Open | - | A106V | [37] | |||||
4AGF | - | DDM | Open | - | L124C | [53] | |||||
4HWA | - | DDM | Open | - | - | [33] | |||||
5AJI | - | DDM | Open | Aliphatic chains | D67C | [32] | |||||
6RLD | 4919 | MSP1E3D1 + Azolecin | Closed | 3 per SU + aliphatic chains | - | [16] | |||||
6PWN | 20508 | MSP1E3D1 + PC:PG (4:1) | Closed | 2 per SU + aliphatic chains | - | [17] | |||||
6UZH | 20959 | Peptidiscs | Closed | - | - | [54] | |||||
6VYK | 2146 | MSP1E3D1 + PC-18:1 | Closed | 1 per SU + aliphatic chains | - | [19] | |||||
6VYL | 21463 | MSP1E3D1 + PC-10 | Subconduct. | - | - | [19] | |||||
6VYM | 21464 | MSP1E3D1 + PC-18:1 + βCD | Adapted (inactivated) | - | - | [19] | |||||
7OO0 | 13003 | DDM | Open | 1 per SU + DDM | - | [18] | |||||
7OO6 | 13006 | DDM + Azolectin | Closed | 1 per SU + DDM | - | [18] | |||||
7ONJ | 12996 | LMNG | Open | 1 per SU + DDM + LMNG | - | [20] | |||||
YnaI | Escherichia coli | 5 | 343 | 0.1 [53] | 5Y4O | 6805 | Amphipols A8-35 | Closed | - | [56] | |
6ZYD | 11557 | DIBMA | Closed | 1 per SU | - | [23] | |||||
6ZYE | 11560 | DIBMA + LPC | Subcond. /unknown | - | - | [23] | |||||
6URT | 20862 | LMNG | Closed | 1 per SU | - | [24] | |||||
7N4T | 24177 | SMA2000 | Closed | 1 per SU | - | [25] | |||||
MSL1 | Arabidopsis thaliana | 5 | 497 | 1.2 [64] | 6LYP | 30017 | digitonin | Closed | - | - | [26] |
6VXM | 21444 | glyco-diosgenin | Closed | n.m. | - | [27] | |||||
6VXN | 21445 | glyco-diosgenin | Open | n.m. | A320V | [27] | |||||
6VXP | 21447 | MSP2NS + soybean polar lipid extract | Closed | n.m. | - | [27] | |||||
FLYC1 | Dionaea muscipula | 6 | 753 | 0.16–0.27 [28,57] | 7N5D | 24186 | glyco-diosgenin | (not fully) open | n.m. | - | [28] |
7N5F | 24188 | glyco-diosgenin | “down” | - | - | [28] | |||||
7N5G | 24189 | glyco-diosgenin | “up” | - | - | [28] |
3. The Force-From-Lipids Principle
4. Coordination of Lipids in MscS and YnaI
4.1. Pocket Lipids
4.2. The ‘Hook’ Lipid
4.3. Pore Lipids
5. State-of-the-Art Models for Lipid-Driven Gating of MscS
5.1. The Hook Lipid as Gating Initiator
5.2. Gating Initiation by Pocket Lipid Removal
6. Modulation of Membrane Properties
6.1. Bilayer Thickness
6.2. Polyunsaturated Fatty Acids
6.3. Cardiolipin
6.4. Cholesterol and Membrane Stiffness
6.5. Lysophosphatidylcholine and Other Amphipathic Compounds
7. Conclusions and Outlook
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Flegler, V.J.; Rasmussen, T.; Böttcher, B. How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels. Int. J. Mol. Sci. 2022, 23, 15071. https://doi.org/10.3390/ijms232315071
Flegler VJ, Rasmussen T, Böttcher B. How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels. International Journal of Molecular Sciences. 2022; 23(23):15071. https://doi.org/10.3390/ijms232315071
Chicago/Turabian StyleFlegler, Vanessa Judith, Tim Rasmussen, and Bettina Böttcher. 2022. "How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels" International Journal of Molecular Sciences 23, no. 23: 15071. https://doi.org/10.3390/ijms232315071