Estimation of SPIO Nanoparticles Uptakes by Macrophages Using Transmission Electron Microscopy
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Macrophage Polarization and Labeling
4.2. TEM Tomography Imaging and Sample Preparation
4.3. Segmentation Method Using the Otsu Threshold
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lysosome Image Number | Total Number of Pixels | Number of Red Pixels | % of Red Pixels | Estimated Number of SPIO |
---|---|---|---|---|
1 | 3654 | 548 | 15% | 93 |
2 | 3782 | 529 | 14% | 88 |
3 | 4140 | 589 | 14.23% | 98 |
4 | 2950 | 502 | 17% | 84 |
5 | 3016 | 431 | 14.32% | 72 |
6 | 3762 | 412 | 11% | 70 |
7 | 2860 | 496 | 17.34% | 86 |
8 | 2700 | 324 | 12% | 40 |
Average | 3358 | 479 | 14% | 78 |
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Aleid, A.; Alhussaini, K.; Almijalli, M.; Saad, A.S. Estimation of SPIO Nanoparticles Uptakes by Macrophages Using Transmission Electron Microscopy. Int. J. Mol. Sci. 2022, 23, 13801. https://doi.org/10.3390/ijms232213801
Aleid A, Alhussaini K, Almijalli M, Saad AS. Estimation of SPIO Nanoparticles Uptakes by Macrophages Using Transmission Electron Microscopy. International Journal of Molecular Sciences. 2022; 23(22):13801. https://doi.org/10.3390/ijms232213801
Chicago/Turabian StyleAleid, Adham, Khalid Alhussaini, Mohammed Almijalli, and Ali S. Saad. 2022. "Estimation of SPIO Nanoparticles Uptakes by Macrophages Using Transmission Electron Microscopy" International Journal of Molecular Sciences 23, no. 22: 13801. https://doi.org/10.3390/ijms232213801