A Novel Mechanism of Immunoproteasome Regulation via miR-369-3p in Intestinal Inflammatory Response
Abstract
:1. Introduction
2. Results
2.1. In Silico Analysis of miR-369-3p Target Genes
2.2. miR-369-3p Directly Decreased PSMB9 Expression
2.3. miR-369-3p Indirectly Regulated Components of the Immunoproteasome Complex
2.4. PA28α and PA28β Subunits Were Decreased by miR-369-3p
2.5. miR-369-3p Reduced Immunoproteasome Activities
2.6. PSMB9 Expression in IBD Patients
3. Discussion
4. Materials and Methods
4.1. Cells Culture
4.2. miR-369-3p Mimic Transfection
4.3. RNA Extraction and Real-Time PCR
4.4. Protein Isolation and Western Blot Analysis
4.5. Immunofluorescence
4.6. Measurement of Immunoproteasome Activity
4.7. UC Specimens and Immunohistochemistry (IHC)
4.8. Bioinformatics and Statistical Analysis
5. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Scalavino, V.; Piccinno, E.; Valentini, A.M.; Mastronardi, M.; Armentano, R.; Giannelli, G.; Serino, G. A Novel Mechanism of Immunoproteasome Regulation via miR-369-3p in Intestinal Inflammatory Response. Int. J. Mol. Sci. 2022, 23, 13771. https://doi.org/10.3390/ijms232213771
Scalavino V, Piccinno E, Valentini AM, Mastronardi M, Armentano R, Giannelli G, Serino G. A Novel Mechanism of Immunoproteasome Regulation via miR-369-3p in Intestinal Inflammatory Response. International Journal of Molecular Sciences. 2022; 23(22):13771. https://doi.org/10.3390/ijms232213771
Chicago/Turabian StyleScalavino, Viviana, Emanuele Piccinno, Anna Maria Valentini, Mauro Mastronardi, Raffaele Armentano, Gianluigi Giannelli, and Grazia Serino. 2022. "A Novel Mechanism of Immunoproteasome Regulation via miR-369-3p in Intestinal Inflammatory Response" International Journal of Molecular Sciences 23, no. 22: 13771. https://doi.org/10.3390/ijms232213771