Immunoglobulin Superfamily Containing Leucine-Rich Repeat (Islr) Participates in IL-6-Mediated Crosstalk between Muscle and Brown Adipose Tissue to Regulate Energy Homeostasis
Abstract
:1. Introduction
2. Results
2.1. Mice Lacking Islr Have Increased Whole-Body Energy Expenditure upon Muscle-BAT Dialogue
2.2. Mice Lacking Islr in BAT Have Improved Energy Expenditure
2.3. Loss of Islr Enhances Mitochondrial Function in BAT
2.4. Secreted Factors Promote Thermogenic Program in Islr-Deficient Brown Adipocytes
2.5. IL-6 Was Identified as an Essential Myokine That Promotes BAT Thermogenesis
2.6. Ndufs2 Interacts with Islr and Is Required for the IL-6 Mediates Thermogenesis
2.7. Deletion of Islr Exerts Beneficial Metabolic Effects in Middle-Aged Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. H&E Staining
4.3. Mitochondrial Isolation
4.4. Western Blot Analysis
4.5. Quantitative Real-Time PCR Analysis
4.6. Brown Preadipocyte Preparation, Culture, and Transfection
4.7. Identification of Secreted Proteins in Mouse Muscle after CTX Injury
4.8. Treatment of Cell Cultures of Mice with IL-6
4.9. Metabolic Cages
4.10. In Vivo Metabolic Assays
4.11. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, C.; Liu, J.; Wang, T.; Su, Y.; Li, L.; Lan, M.; Yu, Y.; Liu, F.; Xiong, L.; Wang, K.; et al. Immunoglobulin Superfamily Containing Leucine-Rich Repeat (Islr) Participates in IL-6-Mediated Crosstalk between Muscle and Brown Adipose Tissue to Regulate Energy Homeostasis. Int. J. Mol. Sci. 2022, 23, 10008. https://doi.org/10.3390/ijms231710008
Liu C, Liu J, Wang T, Su Y, Li L, Lan M, Yu Y, Liu F, Xiong L, Wang K, et al. Immunoglobulin Superfamily Containing Leucine-Rich Repeat (Islr) Participates in IL-6-Mediated Crosstalk between Muscle and Brown Adipose Tissue to Regulate Energy Homeostasis. International Journal of Molecular Sciences. 2022; 23(17):10008. https://doi.org/10.3390/ijms231710008
Chicago/Turabian StyleLiu, Chang, Jin Liu, Tongtong Wang, Yang Su, Lei Li, Miaomiao Lan, Yingying Yu, Fan Liu, Lei Xiong, Kun Wang, and et al. 2022. "Immunoglobulin Superfamily Containing Leucine-Rich Repeat (Islr) Participates in IL-6-Mediated Crosstalk between Muscle and Brown Adipose Tissue to Regulate Energy Homeostasis" International Journal of Molecular Sciences 23, no. 17: 10008. https://doi.org/10.3390/ijms231710008