Genome-Wide Identification of the Ginkgo (Ginkgo biloba L.) LBD Transcription Factor Gene and Characterization of Its Expression
Abstract
:1. Introduction
2. Results
2.1. Composition of the Ginkgo LBD Gene Family and Its Characteristics
2.2. Phylogenetic Analysis and Multiple Sequence Alignment
2.3. Chromosome Localization and Synteny Analysis
2.4. Analysis of Gene Structure and Conserved Motifs
2.5. Ginkgo LBD Promoter Cis-Element Analysis
2.6. Patterns of GbLBD Expression in Different Tissues
2.7. Expression of GbLBD Genes under Different Abiotic Stresses and Different Hormone Treatments
3. Discussion
4. Materials and Methods
4.1. Acquisition of Data and Plant Material
4.2. Identification and Distribution Characteristics of Ginkgo LBD Genes
4.3. Multiple Sequence Alignment and Evolutionary Analysis
4.4. Synteny Analysis and Ka/Ks Ratio
4.5. Analysis of Ginkgo LBD Gene Structure and Conserved Motifs
4.6. Analysis of Cis-Regulatory Elements of the GbLBD Gene Promoter
4.7. Transcription Profiling Based on RNA-Seq Data
4.8. RNA Isolation and Quantitative Real-Time PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tian, Y.; Han, X.; Qu, Y.; Zhang, Y.; Rong, H.; Wu, K.; Xu, L. Genome-Wide Identification of the Ginkgo (Ginkgo biloba L.) LBD Transcription Factor Gene and Characterization of Its Expression. Int. J. Mol. Sci. 2022, 23, 5474. https://doi.org/10.3390/ijms23105474
Tian Y, Han X, Qu Y, Zhang Y, Rong H, Wu K, Xu L. Genome-Wide Identification of the Ginkgo (Ginkgo biloba L.) LBD Transcription Factor Gene and Characterization of Its Expression. International Journal of Molecular Sciences. 2022; 23(10):5474. https://doi.org/10.3390/ijms23105474
Chicago/Turabian StyleTian, Yating, Xin Han, Yanshu Qu, Yifan Zhang, Hao Rong, Kai Wu, and Li’an Xu. 2022. "Genome-Wide Identification of the Ginkgo (Ginkgo biloba L.) LBD Transcription Factor Gene and Characterization of Its Expression" International Journal of Molecular Sciences 23, no. 10: 5474. https://doi.org/10.3390/ijms23105474