A Multi-Perspective Proximity View on the Dynamic Head Region of the Ribosomal 40S Subunit
Abstract
:1. Introduction
2. Results
2.1. Same Complex but Distal Site: A New Reference Point for hr40S SILAC-BioID Experiments
2.2. Proximity Labeling with Biotin at the hr40S from the Rps3 Perspective
2.3. The Asc1-Dependent Microenvironment of Rps3-BirA*
2.4. Another Step Ahead: The hr40S Proxiome from the Rps20-BirA* Perspective
2.5. Toward the Bre5-Specific hr40S Microenvironment with Split-TurboID
3. Discussion
3.1. Regulation of eIF2α Phosphorylation by Gcn2 Might Require an Asc1-Dependent Positioning of Hel2 at hr40S
3.2. The GYF Domain Proteins Smy2/Syh1, the Putative Endonuclease Ypl199c, and the 5′-3′ Exonuclease Xrn1 Localize to the hr40S
3.3. The hr40S Might Be Directly Linked to MAP Kinase Signaling through the MAP4K Ste20
4. Materials and Methods
4.1. Plasmid Construction
4.2. Yeast Strains and Growth Conditions
4.3. Western Blot Analysis
4.4. Proximity Labeling MS Experiments
4.5. Phenotypic Growth Test
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Plasmid Name | Description | Reference |
---|---|---|
pME2785 | MET25Prom, CYC1Term, TRP1, 2 μm | [78] |
pME2787 | MET25Prom, CYC1Term, URA3, 2 μm | [78] |
pME4480 | MET25Prom, CYC1Term, URA3, 2 μm, birA* | [3] |
pME4478 | MET25Prom, CYC1Term, URA3, 2 μm, ASC1-birA* | [3] |
pME4800 | MET25Prom, CYC1Term, URA3, 2 μm, RPS20-birA* | This study |
pME5058 | MET25Prom, CYC1Term, URA3, 2 μm, RPS3-birA* | This study |
pME5059 | MET25Prom, CYC1Term, URA3, 2 μm, FBA1-birA* | This study |
pME5060 | MET25Prom, CYC1Term, URA3, 2 μm, RPL5-birA* | This study |
pME5061 | MET25Prom, CYC1Term, URA3, 2 μm, RPL25-birA* | This study |
pME4984 | MET25Prom, CYC1Term, URA3, 2 μm, NTb-myc | This study |
pME4985 | MET25Prom, CYC1Term, TRP1, 2 μm, CTb-myc | This study |
pME4986 | natPromASC1, CYC1Term, URA3, 2 μm, ASC1-NTb-myc | This study |
pME4987 | natPromASC1, CYC1Term, TRP1, 2 μm, ASC1-CTb-myc | This study |
pME4988 | MET25Prom, CYC1Term, URA3, 2 μm, RPS2-NTb-myc | This study |
pME4989 | MET25Prom, CYC1Term, TRP1, 2 μm, RPS2-CTb-myc | This study |
pFA6a-VN-TRP1 | pFA6a, VN, TRP1, ADH1Term | [77] |
pME5307 | pFA6a, CTb-myc, TRP1, ADH1Term | This study |
pME5312 | MET25Prom, CYC1Term, URA3, 2 μm, RPL5-NTb-myc | This study |
Strain Name | Description | Reference |
---|---|---|
RH2817 | MATα, ura3-52, trp1::hisG | [9] |
RH3510 | MATα, ura3-52, trp1::hisG, asc1-loxP SNR24 | [8] |
RH3493 | MATα, ura3-52, trp1::hisG, Δarg4::loxP, Δlys1::loxP | [73] |
RH3520 | MATα, ura3-52, trp1::hisG, asc1-loxP SNR24, ∆arg4::loxP, ∆lys1::loxP | [73] |
RH3789 | MATα, ura3-52, trp1::hisG, Δbre5::kanMX4 | [18] |
RH3902 | MATα, ura3-52, trp1::hisG, BRE5-CTb-myc::TRP1 | This study |
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Schmitt, K.; Kraft, A.-A.; Valerius, O. A Multi-Perspective Proximity View on the Dynamic Head Region of the Ribosomal 40S Subunit. Int. J. Mol. Sci. 2021, 22, 11653. https://doi.org/10.3390/ijms222111653
Schmitt K, Kraft A-A, Valerius O. A Multi-Perspective Proximity View on the Dynamic Head Region of the Ribosomal 40S Subunit. International Journal of Molecular Sciences. 2021; 22(21):11653. https://doi.org/10.3390/ijms222111653
Chicago/Turabian StyleSchmitt, Kerstin, Alina-Andrea Kraft, and Oliver Valerius. 2021. "A Multi-Perspective Proximity View on the Dynamic Head Region of the Ribosomal 40S Subunit" International Journal of Molecular Sciences 22, no. 21: 11653. https://doi.org/10.3390/ijms222111653