Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways
Abstract
:1. Introduction
2. Results
2.1. Vaspin mRNA and Protein Expression before and after In Vitro Oocyte Maturation, as well as Its Immunolocalisation in COCs
2.2. GRP78 mRNA and Protein Expression before and after Oocyte In Vitro Maturation, as well as Its Immunolocalisation in COCs
2.3. Effect of Vaspin on Nuclear In Vitro Oocyte Maturation and P4 Release in the In Vitro Oocyte Maturation Medium
2.4. Effect of Vaspin on MAP3/1 and PRKAA1 Kinase Phosphorylation in Oocytes and Cumulus Cells after In Vitro Maturation
2.5. Involvement of the MAP3/1 and PRKAA1 Kinases in the Effect of Vaspin on In Vitro Oocyte Maturation
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. COC Collection
4.3. Porcine Oocytes In Vitro Maturation and Experimental Procedure
4.4. Real-Time PCR
4.5. Western Blot Analysis
4.6. Immunofluorescence
4.7. DAPI and Hoechst 33342 Staining
4.8. ELISA Assay
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
EGF | Epidermal growth factor |
FGF | Fibroblast growth factor |
GV | Germinal vehicle |
LH | Luteinising hormone |
FSH | Follicle stimulating hormone |
FCS | Foetal calf serum |
GRP78 | 78-kDa glucose-regulated protein |
P4 | Progesterone |
dbCAMP | Butryl AMP |
PBS | Phosphate-buffered saline |
COC | Cumulus–oocyte complex |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase |
MAP3/1 | Mitogen-activated kinase |
PRKAA1 | AMP-activated kinase |
PVP | Polyvinylpyrrolidone |
CL | Corpus luteum |
BSA | Bovine serum albumin |
PKA | Protein kinase A |
SEM | Standard error of the mean |
STAT3 | Janus kinase |
Oo | Oocyte |
Cc | Cumulus cells |
TBS | Tris-buffered saline |
Meta-I | Metaphase-I |
Meta-II | Metaphase-II |
IVM | In vitro maturation |
IgG | Immunoglobulin G |
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Kurowska, P.; Mlyczyńska, E.; Estienne, A.; Barbe, A.; Rajska, I.; Soból, K.; Poniedziałek-Kempny, K.; Dupont, J.; Rak, A. Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways. Int. J. Mol. Sci. 2020, 21, 9342. https://doi.org/10.3390/ijms21249342
Kurowska P, Mlyczyńska E, Estienne A, Barbe A, Rajska I, Soból K, Poniedziałek-Kempny K, Dupont J, Rak A. Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways. International Journal of Molecular Sciences. 2020; 21(24):9342. https://doi.org/10.3390/ijms21249342
Chicago/Turabian StyleKurowska, Patrycja, Ewa Mlyczyńska, Anthony Estienne, Alix Barbe, Iwona Rajska, Katarzyna Soból, Katarzyna Poniedziałek-Kempny, Joelle Dupont, and Agnieszka Rak. 2020. "Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways" International Journal of Molecular Sciences 21, no. 24: 9342. https://doi.org/10.3390/ijms21249342