Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide
Round 1
Reviewer 1 Report
The authors of this article show that the presence of bioactive hydroxyprolyl peptides in commercially available FBS may mask the effects of Pro-Hyp and Hyp-Gly Supplements. The authors used many citations of their works.
Author Response
Response to Reviewer#1
The authors of this article show that the presence of bioactive hydroxyprolyl peptides in commercially available FBS may mask the effects of Pro-Hyp and Hyp-Gly Supplements. The authors used many citations of their works.
Answer
Our two citations (Ref. 11 and 12 in previous version) were deleted, which do not affect the logic of present study. In addition, we added three new citations on biological function of hydroxyprolyl peptides (Ref 20-22 in revised version).
Reviewer 2 Report
The paper of Asai et al. entitled ”Food derived collagen peptides Pro-Hyp and Hyp-Gly enhance growth of primary cultured mouse skin fibroblasts using fetal bovine serum free from hydroxy prolyl peptide” addresses the effects of Hyp containing collagen peptides on fibroblasts growth. They noticed that FBS masks the effect of the effect of these free peptides.
The paper is well written, but there are some mistakes regarding English language.
I think that the content of this articles is of limited interest for the readers for IJMS but offers some information to the researches involved in fibroblasts.
Author Response
Response to Reviewer#2
The paper of Asai et al. entitled ”Food derived collagen peptides Pro-Hyp and Hyp-Gly enhance growth of primary cultured mouse skin fibroblasts using fetal bovine serum free from hydroxy prolyl peptide” addresses the effects of Hyp containing collagen peptides on fibroblasts growth. They noticed that FBS masks the effect of the effect of these free peptides.
The paper is well written, but there are some mistakes regarding English language.
I think that the content of this articles is of limited interest for the readers for IJMS but offers some information to the researches involved in fibroblasts.
Answer
We believe that the present finding is important for many researchers who use cell culture system, as hydroxyprolyl peptides exert many biological functions. To make it clearer, one sentence with 3 new citations were added in conclusion section.
We carefully corrected English language.
We rewrote some sentences, which were similar to previous report.
